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A: Heat treatments have been used for the reduction of bacterial numbers and even for sterilization…
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A: Microbes are living organisms that are very small and can be seen only with the help of a…
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A: All microbes, such as bacteria, virus, and fungi, requires some essential nutrients for growth. The…
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A: Agar or agar-agar is a jelly-like substances obtained from red algae. Agar is a solidifying agent…
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A: The oxidase is a test used in microbiology to identify bacteria that produce cytochrome c oxidase.…
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A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…
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A: Micro-organisms: The Microscopic universe that widespread in nature as unicellular,…
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A: Asked: Correct option regarding ideal solvent for fat extraction
Q: why it is useful to plot the microbial growth from a growing microbial culture?
A: The term microbial growth refers to the growth of a population of microbe, it is an increase in the…
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A: Ans: Bioreactor It refers to any device that supports the biological environment. This is used for…
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A: Uranium (U) is a radioactive chemical element of the actinoid series of the periodic number with an…
Q: Which of the following statements is true regarding steam sterilization? a. It is a universal…
A: steam sterilization , also called autoclave sterilization , is used in microbiological laboratory in…
Q: The aerotolerance test is used to determine the oxygen requirements for bacterial growth.
A: FALSE.
Q: What are bioreactors ? How are large volume of culture maintained and processed in them ?
A: A bioreactor is a system that enables organisms (such as yeast, bacteria, or animal cells) to…
Q: Under what conditions are media typically sterilized in an autoclave?
A: An autoclave is the pressure chamber used to carry out the scientific and industrial processes…
Q: Besides dehydrated foods, how might the amount of water available to microbes be decreased? Group of…
A: Dehydration is a method that has been used for centuries to preserve foods such as raisins, etc. It…
Q: What are high level disinfectants? What is an example of one? What is the method of action and the…
A: Disinfectants are chemicals that are used to kill the microorganisms present in the surroundings.…
Q: Why is PBS buffer preferred when preparing a monolayer cell culture?
A: PBS (phosphate buffer saline) is a buffer solution which is a water based salt solution containing…
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A: Almost every environment on the planet contains bacteria and microorganisms. In fact, on one square…
Q: If you are purifying a protein from an SEC column, what fractions do you want to collect?
A: SEC stands for Size Exclusion Chromatography. In this process, the mixture of different sizes can be…
Q: what is oil immersion and why is it used
A: Light microscope is a microscopic instrument which uses visible light and magnifying lens to to view…
Q: which method do you think is more reilable, a test utilizing microbial growth media, or a…
A: The investigation of microorganisms is significantly aided by the ability to culture them, that is,…
Q: Of what practical importance are air borne microorganisms to the laboratory workers? What…
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A: Chromatography is defined as the physical method that can be used for separating different…
Q: placing bacteria at ultracold temperatures is as effective at killing microbes as the use of dry…
A: true
Q: What is Bacterial Injury in Microbiology?
A: Bacteria produced in pure culture under ideal circumstances are examined in the lab for their…
Q: If a component is heat sensitive, how might you conveniently achieve sterility of that medium? a.…
A: Option A i.e. filtration
Q: Describe the five phases of a microbial growth curve observed when microbes are grown in a batch…
A: Introduction: Bacteria is unicellular prokaryotic organism that can survive in any kind of…
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A:
Q: For each mode of bioreactor operation listed below, name one advantage and one disadvantage. a.…
A: The three different types of bioreactors have their own set of advantages and disadvantages, one…
Q: What is the meaning of BSC or biological safety cabinet and why is it important and how is it works?
A: Introduction: Biosafety issues refer to the procedures, policies, and principles to be adopted to…
Q: What is a step-by-step process of preparing and inspecting a microorganism under a microscope?…
A: In dark-field microscopy, microbes appear light in a dark background., viruses, macromolecules,…
Q: What medical challenges do biofilms present?
A: Biofilms are microbial colonies that are linked to one another and enclosed in an exo-polysaccharide…
Q: Which is better to use in washing clothes, soap or synthetic detergent? Explain
A: Detergents are the surfactants or the mixture of surfactants. Surfactants are the compounds that…
Q: Please explain the differences between freeze drying,drum drying and air drying and which give a…
A: Freeze drying, drum drying, and air drying are dring methods to remove water content from the cell…
Q: determine the amount of solute and solvent needed to prepare to feeding solution for a 12 hour shift…
A: For calculation the solute and solvent, there are two calculations; ratio proportion and formula…
Q: You are asked to add 2 mL of bacterial culture to 98 mL of water. What is your dilution factor? Show…
A: Introduction :- A test to determine whether you have a bacterial infection is a bacterium culture.…
Q: What are some environmental conditions that must be controlled while purifying a protein?
A: Protein purification is a vital step in biochemistry. It is important for the characterization of…
Q: What could be a suitable limitation for Enzyme and Substrate Concentration Lab
A: Many factors affect the rate at which enzymatic reactions proceed: temperature pH Enzyme…
Q: What is Bioreactor? What are the advantages of Stirred tank Bioreactor over Shake flask.
A: Large-scale production of organic substances such as antibiotics, hormones, vitamins, acids,…
Q: What are two advantages of filtration in microbial control? Explain how the filtration process…
A: According to the question, we have to describe two advantages of filtration in microbial control. In…
Q: Which one of the below given biomolecules can serve as the immobilized layer on biomaterials…
A: Biotine serve as the immobilized layer on biomaterials surfaces. Among the given options of Human…
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- 3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTESHello sir, please, I want a simple talk about this subject, I understand it means abbreviation. Polymeric Biomaterial ОВЈЕСТ: Definition, classifications and application of polymeric biomaterials. IntroductionDefine about sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)? Explain importance of sodiumdodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ?
- Discuss the principles and applications of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in molecular diagnostics.Given this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution. Given that each bacterial…Discuss “Qualitative vs Quantitative tests for amino acids and proteins”. Discuss “Qualitative vs Quantitative biochemical analysis”. Explain quantitative tests widelyused in biochemical analysis.
- Name the three basic classes of drug targets in medicinal chemistry and indicate, which are the most/least important as targets for biologics? I don't sure if I answer these questions correctly. Hope someone can give me confirmation and explain to me how enzymes will be affected by bioloigcs? My answer so far: The three common classes of drug targets are; receptors, nucleic acid, and enzymes. As a target for biologics, the least important will be a nucleic acid, and the most important will be receptor and second enzymes. Biologic drugs such as monoclonal antibodies will bind to the antigens, which are overexpressed receptor that need to be deactivated. In this case, the monoclonal antibodies will act as an antagonist and block the ligand from binding to the antigens, which are extensively presented in cancer cells. In addition, it can also affect enzymes by.......?List three coating techniques used to make sol-gel coatings, and briefly describe each technique. Need answer in short and ASAPWhat are the disadvantages of biochemical test?
- State the principle that underlies the following biochemical tests: a. Voges-Proskauer testA biochemist discovers and purifies a new enzyme, generating the purification table below. Procedure Total protein (mg) 20,000 5,000 4,000 Activity (units) 4,000,000 3,000,000 1,000,000 800,000 750,000 675,000 1. Crude extract 2. Precipitation (salt) 3. Precipitation (pH) 4. Ion-exchange chromatography 5. Affinity chromatography 6. Size-exclusion chromatography From the information given in the table, calculate the specific activity of the enzyme after each purification procedure. b. Which of the purification procedures used for this enzyme is the most effective (I.e. gives the greatest relative increase in purity)? Which of the purification procedures is least effective? d. Is there any indication based on the results calculated in part A that the enzyme after step 6 is now pure? What else could be done to estimate the purity of the enzyme preparation? 200 50 45 a. C.Nanoparticles were added in a liquid to prompt the medication release, and it was observed that 5 mg of the medication was released in 1 liter of PBS, which accounted for 60% of the total amount of the drug. While producing an equivalent number of nanoparticles, 5 ml of 10 mg ml−1 of the drug was added. What is the efficiency of encapsulation?
