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give the respective the TOTAL DILUTION FACTOR of respective serial dilutions. show complete solution.
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- BONUS (15 points) The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2. étv A) F12 F9 F10 F7 % & %3D delete 5 { T Y J KEcoRI 4359 Aatll - Zral 4284 Bei 4209 BsrBl 4205 Clal - BspDI 23 Hindill 29 EcoRV 185 Bmtl - Nhel 229 Sspl 4168 Earl 4155 Acul 4048 Xmnl 3961 Hincll 3905 Scal 3844 BamH 375 Sgrl 409 Banll 471 Banll 485 Вы 3787 Bsl 3759 Bgl 528 Sphl 562 EcoNI 622 Sal - Acct - Hincll 651 Pvul 3733 Pstl 3607 Bartl 3602 Pshl 712 Asel 3537 Eagl 909 Bell 949 Bsal 3433 BarDI 3420 Nrul 972 PBR322 4,361 bp BstAPI 1045 Ahdi 3361 BspMI - Bfual 1063 PAMI 1315 Bsml 1353 PAMI 1364 Acul 3000 Ori Styl 1369 Aval - BsoBl 1425 PpuMI 1438 Msel 1444 Bigl 1447 Ppu 1480 AlwNI 2884 Bell 2777 kI 2682 rop Drdi 2575 Bsgl 1650 BspEI 1664 Pcil - Afl 2473 rBl 2404 Earl 2351 Bspol - Sapl 2350 Ndel 2295 BstAPI 2291 Bsaß 1668 Xmat 2029 Pvull 2064 BsmBI 2122 Dndl 2162 BstZ171 - Acct 2244 Bsal 2225 TthI- PI 2217 Figure 1 If your gene of interest was inserted at the Sphl restriction site of the plasmid illustrated in Figure 1, describe the screening process to select the positive recombinants.d-6f06-490d-b3d3-cb18767c100 Post-Lab: Streak Plate Isolation Homework. Due in 12 hours 2/5 answered Unanswered Post-Lab: PIV 2.5 Homework Unanswered How do colonies of E. coli differ from those of P. mirabilis? inancwarnd
- TUBE COLOR ture Bottle llov tigh R Go Bl r d ir r e J TYPE Whole Whole Blood culture Culture bacteria, some viruses MA10 Week 3 CLIA Waved Test Box Research each of the below CLIA Waved Test and provide 1 to 2 examples of a test that will fall under the heading and any common diseases that are identified with each test. 1. CLIA-waived hematology test 2. CLIA waived chemistry test 3. CLIA waived urinalysis 4. CLIA waived immunology test CH 43 PP 5. CLIA waived microbiology testWhich of the following is the a-anomer? H но H Но H2C H ОН H2C- нн ОН со 4 1 2 1 Submit 3 -OH Н ОН -OH ОН Н there is no a-anomer ОН ОН Н Request Answer H Но НО Н H2C нн ОН 2 H2C ОН -OH 4 Н ОН н н H OH -О. ОН ОН Н ОН НEsc F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 40 %23 5. What is the physical appearance (solid, liquid, semi-solid) of the following media: broth tube, agar slant tube, agar deep tube, and agar plate. What are you testing when you introduce an inoculum with a needle on an agar deep tube? at
- SATestprep, LLC- Online State es/test/tq.php?testid-9848strandid=4936&element=D&assignment id-41828865&load test=18teacherPreview=#test A cellular protesses, maximiZing cIcIency. The ENDOSYMBIOTIC THEORY 3nascond edybee pel, he eady nukarycte ponsuned otosuhetc ngs n the plas membrane t nn ses pokaryote ge me to endonmbe comporen Incluting anuceus ns endpl um baderis roued int Endplaun cke Nudes Phot etic baceriam Modern photosynthetic ukaryote Proto-ukaryote -Meochondrim Atec bacenum Anetredon even te ancesia kaye conumed ae bic bactia fat evoived eto mnodhonca Modern heteotrophic eukanyote The diagram provided shows the evolution of membrane-bond organelles in eukaryotic cells. The Endosymbiotic Theory proposes that the organelles distinguishing eukaryote cells evolved through symbiosis of individual single-celled prokaryotes. Which of these statements provides supporting evidence for the endosymbiotic theory? A) Chloroplasts have a single phospholipid membrane. B) The DNA in…Helping tags: Biology, bacterial count, dilution, serial dilution WILL UPVOTE, just pls help me answer the following questions and explain them clearly. Pls show complete solutions also for the computation part. Thank you. 1. A bacterial culture was grown for 9 hours. At 3-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.85% NaCl. Three consecutive dilutions were further made by using 1 ml aliquot in 9 ml of 0.85% NaCI. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. Sampling COUNTS 2nd dilution 3rd dilution 4th dilution 0,0 30: 35 250;245 1st dilution 1st 2nd 3rd 0; 0 240; 235 TNTC 55; 60 5; 6 TNTC TNCT TNTC TNTC a) Illustrate the dilution series used and label the final dilution of each dilution. b) Determine the bacterial count (CFU/ml) every 3 hours of incubation for 9 hours. Show all computations.Average plaques for bacteriophage A，B，C are 137，36，25. PFU/ml is average plaques multiply by the volume dilution multiply by the dilution factor. Show your working for each bacteriophage.
- WT E. coli B Lysate titer on B K pfu/ml 10⁹ 10⁹ 0.1 m.u. 0.2 m.u. 0.5 m.u. 1.0 m.u. rll#x 2.0 m.u. E. coli B Lysate titer on B K 10⁹ 103 ril#y E. coli B Lysate titer on B 10⁹ K 10² rll#x E. coli B Mutations rll#x and rll#y are separated by how many map units? Lysate titer on ril#y B K 10⁹ 107Font Paragraph Styles 12. What class of Biosafety Cabinet is shown below? C A I Room air Contaminated air HEPA-liltered air Side ViewThe student advises using the same selection procedures and inoculating a 5 mL overnight culture with the transformed cells instead of searching for transformed coli on LB agar plates in order to save time. Why isn't this a wise decision?
