What information can be obtained from the slides prepared by wet mount and hanging drop technique for microscopic observation? Give at least three (3) information. What are the limitations of each method. (Provide 2 limitations)
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What information can be obtained from the slides prepared by wet mount and hanging drop technique for microscopic observation? Give at least three (3) information. What are the limitations of each method. (Provide 2 limitations)
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- What information can be obtained from the slides prepared by the wet mount technique and the hanging mount technique? Give at least three (3) information. What are the limitations of each method (Provide two limitations)Describe the key components and principles of operation of a x-ray unit used for film/screen mammography.Use diagrams,where appropriate to illustrate your answerHere are the materials and method for the basic spectrophotometer experiment Materials:1. Paper template2. Scissor3. Light source (i.e. torchlight, portable light)4. Paperboard (black colour)5. Unused compact disc (CD)6. Camera detector (i.e. webcam, laptop, mobile phone) Methods: 1. Make a foldable paper spectrophotometer using the paper template and followthe instruction as in https://publiclab.org/sites/default/files/8.5x11minispec3.8.pdf2. Then, mounted the paper spectrophotometer to the camera detector.3. Download any freely available spectral analyzer.4. Test and observe the wavelength with and without the presence of a light source.5. Finally, observe the wavelength with a different transparent colour sheets (you canchoose any colour). Troubleshooting/ problem(s) encountered for basic spectrophotometer experiment.
- Complete the following table by calculating the total magnification of the different objectives of the microscope with the given eyepieces. OBJECTIVES OCULAR Designation Magnification Focal Length Numerical Aperture (NA) Final magnification with: 10x 15x 25x Scanner 4x 46mm 0.10 LPO 10x 16mm 0.25 HPO 40x 4mm 0.55 OIO 100x 1.8mm 1.30What are the differences between scanner, low power objective, high power objective and oil immersion objective? Introduce the terms stated above and explain comprehensively.Complete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)
- Assume your field of view under a 3X objective is 6mm across. What is the field of view in mm under a 20X objective? Show clear work with units for credit. Field of view 20X objective? ___mm What is the field of view of this 20X objective in micrometers? If you observe a specimen under the 20X objective and the specimen spans one-quarter of the field of view distance, how long is the specimen in micrometers?Which of the following statements is/are TRUE? Kindly choose all that applies. Select the correct response(s): When FOV increases, the specimen magnification decreases. When FOV increases, the light intensity increases. When FOV increases, the specimen magnification increases. When FOV increases, the light intensity decreases.The FOV on low power of a microscope is measured to be 4.5mm. Using the chart below and the above listed formulas calculate: FOvx = FOvlow X Maglow Magx S.S. = FOvx # times the specimen fits across the FOv Total Mag = Ocular lens x objective lens a) What is the FOV on High Power? (Magnification on High power is 40x) Can you please include the units too. asap please.
- Write T (True) or F (False) for the following statements. 1. The objective lens is the one nearest to the observer's eye. 2. Only special lens paper should be used to clean the objectives, oculars, and the condenser of the microscope. 3. The working distance is the distance between the specimen and the objective lens. _4. Magnifying a blurred image generally reveals further details. 5. When a microscope is parfocal, you should not have to use the coarse adjustment at higher magnification. _6. The total magnification of the object seen at high dry power is approximately 40X. 7. Immersion oil can be used to increase resolution of all the objective lenses in a brightfield microscope. _8. Always be sure to oil your microscope lenses before returning the instrument to its designated space. 9. Animals will not survive without mitochondria because they will not be able to make ATP. 10. The use of normal saline solution (NSS) in viewing cheek cells is to prevent lysis or crenation of cells.What factors must be addressed to properly acquire quantitative fluorescence microscopy images? What information about the imaging system should be recorded? What controls are needed and what standards are available?You are asked to calibrate the ocular micrometer for the 4x objective using the stage micrometer. You observe 32 stage micrometer units line up with 15 ocular micrometer units. What is the value for each division of the ocular micrometer for this objective? (Round to the 1 decimal place)