Give the chemical principles involved in a positive Biuret test, Millon’s Test and Sakaguchi Test. What is the chemical basis of the Ninhydrin Test, Hopkins – Cole test and Sulfur reaction test? What chemical structure should be present in an amino acid or protein to give a positive Xanthoproteic test?
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- Give the chemical principles involved in a positive Biuret test, Millon’s Test and Sakaguchi Test.
- What is the chemical basis of the Ninhydrin Test, Hopkins – Cole test and Sulfur reaction test?
- What chemical structure should be present in an amino acid or protein to give a positive Xanthoproteic test?
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- Discuss the medical application of the Benedict’s test? What other test(s) are used in parallel to Benedict’s test?what are the Amino Acids that test positive for Ninhydrin test? what is the principle of detection?What chemical structure should be present in an amino acid or protein to give a positive Xanthoproteic test? What chemical test could be used to differentiate a protein from an amino acid? Explain.
- Please explain the theoretical background (positive color reactions and what is being detected) involved in the different qualitative test for proteins that has been isolated intact or hydrolyzed. Please directly answer the theoretical background (positive color reactions and what is being detected) of each test GENERAL: BIURET - NINHYDRIN SPECIFIC XANTHOPROTEIC TEST - MILLON'S TEST - HOPKINS-COLE TEST - SAKAGUCHI TEST - LEAD ACETATE TEST - NITROPRUSSIDE TEST - FOLLIN'S REACTION - SULLIVAN'S TEST - PAULY REACTION - LIEBERMAN'S TEST -The table below summarizes the results for Millon's test. Provide the correct remarks from the results if Negative for Millon's Test or Positive for Millon's Test and if Contains phenolic hydroxy group or Does not contains phenolic hydroxy group.What is the purpose of Durham test ?
- What is the chemical basis of the Xanthoproteic Test? Give the chemical reaction involved.What is the purpose of Biuret’s test? In biuret’s test, does the color in each test tube containing the sample vary? Why or why not?Please explain the theoretical background (positive color reactions and what is being detected) involved in the different qualitative test for proteins that has been isolated intact or hydrolyzed. Please directly answer the theoretical background (positive color reactions and what is being detected) of each testNITROPRUSSIDE TEST - FOLLIN'S REACTION - SULLIVAN'S TEST - PAULY REACTION - LIEBERMAN'S TEST -