Q: Describe how an appropriate inoculum can be usedto successfully start an enrichment culture.
A: Enrichment culture is culturing a specific microorganism using certain growth media that favors…
Q: What advantages do solid media offer for the isolation ofmicroorganisms?
A: Micro-organism are those that cannot be seen by the naked eye, but are visible under microscopes.…
Q: Why does a precipitate form on Sierra’s medium when inoculated with a lipolytic organism? Discuss…
A: Nutrient mediums are used to provide growth to the microorganism in invitro experiments. These…
Q: Define agar.
A: A dried jelly-like substance called agar, commonly referred to as agar-agar, is made from the red…
Q: Why is dilution a necessary part of pure culture preparation?
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: Why are biochemical tests used to identify microbes?
A: Microbes are living organisms that are very small and can be seen only with the help of a…
Q: Why is CFU more applicable to a culture of streptococcus than to a culture of E.coli
A: Streptococcus is a Gram-positive bacteria that are spherical in shape. This bacteria is responsible…
Q: Why is it important to keep the culture at the correct incubation temperature when measuring the…
A: Generation time is the time which is taken by the bacteria to double in number during a specified…
Q: Why is it important for a microbiology laboratory technician to use aseptic technique?
A: The majority of the microbes onto which scientists work in laboratories are known pathogens.
Q: Why is it necessary to maintain aseptic conditions in microbiology?
A: Aseptic conditions means microbs free environment. Microbs means bacteria, virus and fungi. It may…
Q: Define sterilization.
A: Microbial control indicates the prevention or inhibition of microbial growth. It involves physical…
Q: discuss the significance of the different aseptic techniques utilized in maintaining sterility of…
A: The laboratory that carries out growth of prokaryotic cells or eukaryotic cells in physiological…
Q: what type of bacteria would be used to make sauerkraut?
A: Sauerkraut is product of cutting raw cabbage that fermented by various lactic acid bacteria. The…
Q: micro-organisms are the most resistant to heat sterilization?
A: Most of the bacteria cannot survive adverse heat condition. But somehow particular bacteria can…
Q: Which of the following statements is true regarding steam sterilization? a. It is a universal…
A: steam sterilization , also called autoclave sterilization , is used in microbiological laboratory in…
Q: Under what conditions are media typically sterilized in an autoclave?
A: An autoclave is the pressure chamber used to carry out the scientific and industrial processes…
Q: CTSA growth medium designed
A: Under the regulated conditions of the laboratory when microbial cells are allowed to grow and…
Q: Explain why sulfa drugs prevent bacterial growth without harming the human host.
A: Antimicrobial drugs are those which destroy or inhibit the growth of microorganism. They have their…
Q: Why are semisolid media sometimes preferred over slide techniques for evaluating bacterial motility?
A: Semi-solid media are soft media that is used in demonstrating the motility of bacteria and…
Q: Why should culture media after inoculation be incubated at an optimal temperature immediately?
A: Culture media, also called as growth media, are particular combinations of nutrients and other…
Q: What advantages do solid media offer for the isolation of microorganisms?
A: “Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: Why is PBS buffer preferred when preparing a monolayer cell culture?
A: PBS (phosphate buffer saline) is a buffer solution which is a water based salt solution containing…
Q: If you had a mixed culture that contained a Gram-negative enteric bacterium and Gram-positive…
A: MSA or mannitol sale agar used in microbiology. It encourages growth of some group of bacteria…
Q: Why is it desirable that most cultures be inspected after 15 to 18 hours of incubation?
A: Introduction Microbial culture is a microbiological technique that involves actively multiplying…
Q: Explain why it is important to use only a small amount of bacteria when preparing a smear
A:
Q: What are some advantages and disadvantages of using ionizing radiation to sterilize food?
A: Sterilization involves physical methods for removing or controlling the microbes. The physical…
Q: What settings and time are normally used for sterilization in an autoclave?
A: The fundamental rule of steam sterilisation as cultivated in an autoclave, is to open every thing to…
Q: which method do you think is more reilable, a test utilizing microbial growth media, or a…
A: The investigation of microorganisms is significantly aided by the ability to culture them, that is,…
Q: Heat-labile material to be introduced to culture medium was filtered using membrane filters with…
A: given that the heat labile material which is introduced to culture media is filtered using a…
Q: What makes agar good support for microbial growth?
A: Microbial culture is a method of multiplying microbial organisms by letting them reproduce in…
Q: Why must water be subjected to microbiological analysis?
A: Water is used for various purposes in daily lifestyle including food. Thus, it should be monitored…
Q: Distinguish heat sterilization and "commercial sterility".
A: lowering the prevalence of harmful bacteria until they are no longer a health threat. usually…
Q: Why is 15 minutes generally used for sterilization? Why is agar added to medium?
A: Sterilization is the process in which all life forms like microbes (fungi, bacteria, viruses,…
Q: How can moist heat sterilization can sterilize antibiotic solution?
A: Moist heat sterilization is a technique that uses heated, increased pressure steam to sterilize an…
Q: What is the most difficult type of microbial cell or structure to kill? What methods can be used to…
A:
Q: Discuss how can some anaerobes be maintained in pure cultures?
A: Introduction :- A pure (or axenic) culture is a population of cells or multicellular creatures that…
Q: How can organisms develop resistance to chemical disinfectants?
A: Bacteria can develop resistance to disinfectants over time either by acquisition of exogenous mobile…
Q: What products are sterilized using high energy ionizing radiation?
A: Sterilization is the process of killing microorganisms.
Q: Why do we grow the bacteria in minimal media when testing for citrate utilization
A: Minimal media are those that provide the minimal nutrients possible for colony development, usually…
Q: Why are plated media stored in an inverted position?
A: Plated media enables the isolation of microorganisms from samples or specimens.
Q: Write the limitations of sterilization by Dry heat?
A: The dry heat sterilization technique includes the removal or killing the microorganism and require…
Q: What method can be used to achieve sterilization?
A: It is required to describe the method to be used to achieve sterilization.
Q: Why is chlorination, although beneficial in terms of bacterial pathogen control, of environmental…
A: Chlorination is a disinfection procedure in which microorganisms are killed by the use of chlorine…
Q: Suppose your Professor handed you a test tube with 2.0mL of E.coli broth culture and told you to…
A: In both basic and applied research, "microbiology" is a highly significant area. Proteomics,…
Explain why it is essential that bacteria cultures be sterilized in an autoclave.
The microbial cultures are grown in the laboratory so as to multiply them or increase the number of the cells in the culture medium under controlled conditions. To grow the cells, culture media is mixed with agar and poured in the Petri plates to solidify. Then agar plates are used as a solid medium for the growth of microbes.
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- Why are complex media preferable to chemically defined media for routinecultivation of microorganisms?What advantages do solid media offer for the isolation of microorganisms? How will you check the purity of an isolate?Outline the different physical and chemical methods of sterilization. Briefly describe how each method control microbial growth
- Explain the significance of aseptic culture technique in growing microorganisms.discuss the significance of the different aseptic techniques utilized in maintaining sterility of the cell culture lab.Listen Which of the following methods of microbial control is used most often with surgical equipment? a) autoclave b) sugar and salting c) dessication d) lyophilization