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Explain the working principle of Atomic Absorption Spectrophotometer and Draw the general Diagram of it.
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- Define absorption spectrum and action spectrum, and describe the purpose of using a spectrophotometer to determine the absorption spectrumExplain why is absorption used for studying the dyes, while fluorescence is used for studying the quantum dots?Assume that you have an “X” solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. Prepare a standard curve by using the absorbance of known concentrations (Hint: you can learn how to prepare a standard curve by using Excell from youtube). Calculate the concentration of unknown.
- Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's LawExplain the relationship between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.Describe the mechanism of SERS( Surface Enhanced Raman Spectroscopy).
- The electronic excitation method in fluorescence and absorption spectroscopy is the same. What are some of the variations between using a fluorimeter to perform an excitation scan and an absorption scan? Do you think the two spectra should be similar? Describe. More info attached like protocol if neededWhat is the main application of Atomic absorption spectrophotometer (AAS)?if the absorbance value measured in a spectrophotometer is 0.5, how much of the original incident light is being measured by the detector?
- What should be the minimum specification of a spectrophotometer that will be used to determine the absorption maximum (???? ) of any compound? What could be possible outcomes if a compound is not measured in the wavelength of its ????? I'm having a hard time researching about this.Attached references are much appreciated.In the context of flow cytometry data: The Lactobacillus cells also show a clear increase in PerCP (red) fluorescence when stained with SYBRGreen (SG) compared to unstained cells. Why might this be the case? Think about the emission spectrum of chlorophyll and SG in combination with the dichroic mirrors (e.g. the ‘filters’ for the detectors).Discuss the types of interferences in atomic absorption spectrophotometry.