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- Consider a bi convex lens with radius of curvatures |R1| = 30 mm and |R2| = 180 mm, ct = 5 mm and glass is N-BK7. This is used as camera lens in order to image a man who is at 1 km away from the camera. We want to resolve 10 cm on his body. Assume that λ = 550 nm. Using zemax, determine the MTF value for the resolution of R = 80 lp / mm in image plane which is placed at paraxial focus. (Hint: First, find the diameter of the lens) Which one: a.32.1 % b.28.7 % c.12.3 % d.58.5 % e.46.8 % f.57.2 % g.20.9 % h.15.6 %Explain the underlying physical principles in the creation of image contrasts for the following two processes of image contrasts for the following two procedures. (a) Magnetic resonance tomography (b) X-ray-computed tomographyWhat do you think is/are the characteristic of sulfamethoxazole (antibiotic) that allows it to absorb light in a spectrophotometer at a UV range or at a colorless wavelength? Please provide detailed and clear responses. Thank you very much!
- Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's LawHow can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a colorless sample? Please answer in detail and clearly. Thank you so much!Objective lens X has a limit of resolution equal to 0.2 mm while objective lens Y has 30 µm. a. Which among the two can magnify a mosquito wing in finer detail? b. Which among the two would show a greater area of the mosquito wing?
- Identify all accurate statements: Using a neutral excitation filter can reduce photobleaching. However… A.This can reduce the fluorescence emitted. B.This only delays photobleaching if exposure to light is prolonged. C.This can introduce aberrations in the image. D.This may be toxic to the sample to be observed.“Atomic absorption spectroscopy (AAS) and UV-Vis spectroscopy is similar in principles”. Do you agree with this statement? Explain briefly.Sulfamethoxazole is a type of sulfonamide antibiotic. Its absorbance ranges from 250-300 nm in a UV spectrophotometer, which tells that it is in the UV region. What characteristic/s of sulfamethoxazole is responsible for the absorbance in the UV region? Please answer in detail and clearly. Thank you!
- Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?What did you notice about the Scalp Bald Human photomicrographs shown at (a) 40x vs (b) 100x TM? Why do you think your instructor is asking you to look at it at two different total magnifications?The basic component of a fluoroscopic x-ray system suitable for interventional procedures consist of a generator,x-ray tube and housing including collimator and filtration,patient table,anti-scatter grid and an image receptor.Describe the key design features and function of each of these component and briefly discuss the role of automatic brightness control