An amino acid on the surface of an enzyme was labelled covalently with 1.5-I AEDANS and it is known that the active site contains a tryptophan residue. The fluorescence quantum yield of tryptophan decreased by 15 per cent due to quenching by 1.5-I AEDANS. What is the distance between the active site and the surface of the enzyme?

An amino acid on the surface of an enzyme was labelled covalently with 1.5-I AEDANS and it is known that the active site contains a tryptophan residue. The fluorescence quantum yield of tryptophan decreased by 15 per cent due to quenching by 1.5-I AEDANS. What is the distance between the active site and the surface of the enzyme?

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter19: Nuclear Magnetic Resonance Spectroscopy

Section: Chapter Questions

Problem 19.17QAP

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