Draw these steps of Qualitative Slide Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR: SCREEN AND TITER 1. Bring all reagents and test samples to room temperature before use. 2. Make a 1:20 dilution of the patient serum by pipetting 0.1 ml of patient serum into 1.9 mL of 1X diluent or saline in a test tube. Pipet 50 µl of the diluted sample onto one test circle on the slide. Place one drop (about 50 mL) of positive control onto a separate test circle and one drop of negative control onto another test circle. 4. Invert the latex reagent bottle several times to mix the beads into the suspension. Add one drop of well-shaken reagent to each test area of the slide. 5. Mix each test area with a disposable stirrer, spreading each mixture over the entire section. Use a clean disposable stirrer for each test area. 6. Rock the slide gently with a rotary motion for 2 minutes and observe immediately for agglutination.
Draw these steps of Qualitative Slide Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR: SCREEN AND TITER 1. Bring all reagents and test samples to room temperature before use. 2. Make a 1:20 dilution of the patient serum by pipetting 0.1 ml of patient serum into 1.9 mL of 1X diluent or saline in a test tube. Pipet 50 µl of the diluted sample onto one test circle on the slide. Place one drop (about 50 mL) of positive control onto a separate test circle and one drop of negative control onto another test circle. 4. Invert the latex reagent bottle several times to mix the beads into the suspension. Add one drop of well-shaken reagent to each test area of the slide. 5. Mix each test area with a disposable stirrer, spreading each mixture over the entire section. Use a clean disposable stirrer for each test area. 6. Rock the slide gently with a rotary motion for 2 minutes and observe immediately for agglutination.
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter4: Basic Immunology And Immunohematology
Section4.3: Tests For Rheumatoid Factors
Problem 2.1CS
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Transcribed Image Text:Draw these steps of Qualitative Slide Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF
RHEUMATOID FACTOR: SCREEN AND TITER
1. Bring all reagents and test samples to room temperature before use.
2. Make a 1:20 dilution of the patient serum by pipetting 0.1 ml of patient serum into 1.9 ml of 1X
diluent or saline in a test tube. Pipet 50 µul of the diluted sample onto one test circle on the
slide.
3. Place one drop (about 50 mL) of positive control onto a separate test circle and one drop of
negative control onto another test circle.
4. Invert the latex reagent bottle several times to mix the beads into the suspension. Add one
drop of well-shaken reagent to each test area of the slide.
5. Mix each test area with a disposable stirrer, spreading each mixture over the entire section. Use
a clean disposable stirrer for each test area.
6. Rock the slide gently with a rotary motion for 2 minutes and observe immediately for
agglutination.
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