1. In Xanthoproteic test the appearance of pink color confirms the presence of proteins. A. True B. False 2. Calamansi juice can retard/prevent browning in fruits and vegetables. A. True B. False
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- 1. Albumin will give positive result to Biuret test. A. True B. False 2. Tyrosine will give positive result to Xanthoproteic test. A. True B. FalseDraw the steps of Qualitative Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR 1. Bring the test reagents and samples to room temperature (Note 1). 2. Shake the latex vial gently. Aspirate dropper several times to obtain a thorough mixing. 3. Place 1 drop (50 µL) of the serum under test into one of the circles on the card. Dispense 1 drop of positive control serum and 1 drop of negative control into two additional circles. 4. Add 1 drop of ASLO-Latex Antigen to each circle next to the sample to be tested. 5. Mix the contents of each circle with a disposable stirrer while spreading over the entire area enclosed by the ring. Use separate stirrers for each mixture. 6. Rotate the slide by means of a mechanical rotator (100 r.p.m.) for a period of 2 minutes (Note 2). 7. Observe immediately under a suitable light source for any degree of agglutination.i. The 21 Day (or 3 Weeks) Cumulative Irritancy Patch test ii. The Repeat-insult Patch Test Describe the procedure of the two tests and the interpretation of their results for the presence of allergies.
- 2. Provide information about the TLI test.1. define the latex agglutination (LA) and gelatin particle agglutination tests (GPAT) techniques 2. State the principle underlying the above techniques 3. state the types or forms of the techniques if present4. What is the rationale behind the sequence of the order of draw? Sterile tube (blood culture) a. b. Blue-top coagulation tube C. Serum tube (glass) with no additive d. Serum tube with clot activator, with or without e. Heparin tube with or without gel plasma separator f. EDTA tube g. Glycolytic inhibitor tube tubo with silica clc
- 1. Compare and contrast DAT from IAT based on procedure and clinical significance. 2. Explain the importance of the three phases of Indirect Coomb's test. 3. Differentiate the three potentiators used in Coomb's Test (PEG, LISS, 22% BSA)II. Two Tier Test. Encircle the letter corresponding to your chosen answer then rephrase the chosen statement to make it correct. 1. Which is not true of the spleen? a. The fetal spleen produces RBC. b. Antibodies are produced in the spleen by cells called fixed monocytes. c. The spleen is located in the LUQ of the abdomen. d. Fixed macrophages in it phagocitize pathogens in the blood. Rephrase: 2. Which is not true about the lymph nodes? a. There are three major groups: cervical, axillary and inguinal. b. During serious infection, they become swollen. c. They are located along the lymphatic ducts. d. They contain fixed macrophages and plasma cells. Rephrase: 3. Which is not true regarding thymus and immunity? a. The thymus is most active in the fetus and child. b. The thymus is located above the thyroid gland. c. T cells are the lymphocytes produced by the thymus. d. The thymus is necessary for T cells maturation. Rephrase:REACTIONS OF CARBOHYDRATES Reagents:Samples:10% glucose, galactose, fructose, xylose, maltose, sucrose, lactose, starch (5 ml each) ,2 ml Fehling's A, 2 ml Fehling's B, 8 ml Benedict's reagent, 8 ml Barfoed's reagent, 3 g sodium acetate, 2 g phenylhydrazineHCl Materials: 12( 20 ml) test tubes, test tube rack, test tube brush, test tube holder, alcohol lamp, tripod, wire gauze, water bath, (10 ml) graduated cylinder, dropper, stirring rod, beaker(250 ml), spatula, microscope,7 glass slide, 7 cover slip, watch glass, platform balance Procedure: 1. Osazone formation –Mix 3 g sodium acetate and 2 g phenylhydrazineHCL and 14 ml distilled water. Warm with stirring until solution clears. Place 1 ml sugar solution (glucose, fructose, galactose, maltose, xylose, sucrose, lactose) in separate tube. Add 2 ml of the hot solution in each of the sugar solution, stopper with cotton, mix well and heat in a boiling water bath for 30 minutes. Cool at room temperature and examine the crystals under the…
- 5. The MLT performs patient testing against specific reagent anti-sera and obtains the following results: Anti-e Anti-D 4+ Anti-c 3+ 3+ Anti-C 3+ a) What is this patient's Rh phenotype? Anti-E 0Horseradish essential oil is not generally considered suitable for use with ICU patients although, according to research undertaken by Nedorostova et al 2011, it has proven effective against: Choose one answer. a. MRSA b. heart attack c. stroke d. sepsis4. D-dimer screening test is important in disseminated vascular disorders (DIC) diagnosis. Why is this statement correct? or wrong? 5. Lupus anticoagulants (LA) prolong APTT results. Yes or No? Defend your answer.