Determine the microbiology tests used to identify the microorganism(cutaneous anthrax) i. Gram stain Culture results Colony characteristics on media Diagnostic test results that identified the microorganism
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Determine the
i.
- Culture results
- Colony characteristics on media
- Diagnostic test results that identified the microorganism
Cutaneous anthrax is the most common form of anthrax infection, and it is also considered to be the least dangerous kind of infections. Cutaneous anthrax is most common on the head, neck, forearms, and hands. It affects the skin and tissue around the site of infection.
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- Identify: 1. Cell shape and arrangement in A? 2. Gram stain reaction in A? 3. Cell shape and arrangement in B? 4. Gram stain reaction in B? 5. Genus and species of B? (component of the normal flora of the skin)Give the procedure of Sabin-Feldman dye test. Describe the positive and negative results.Mention any three (3) fluids used in cleaning lenses in the microbiological laboratory.
- For Staphylococcus aureus, indicate which tests need to be done & what media you need. Fill in box provided Test Media NeededIdentify what microorganisms are in each of the 10 plates from studying the gram stain and agar plates，and if they have any known antimicrobial resistanceOven sterilization of aqueous culture medium. Effect: Rationale:
- An outbreak of Methicillin-resistant Staphylococcus aureus (MRSA) in the surgical ward at the hospital forces the hospital administrator to cancel and postpone all non-essential surgeries until the source of infection has been located and for measure put into place. Bacterial samples obtained from swab of wad staff equipment and surfaces arr brought to the lab for analysis. 1. What testing method will you employ to determine the level of exposure? 2 How would a positive test present? 3. How would a negative test present? 4. What recommendations would you make in your report?Why are specimens for bilirubin testing protected from light? Discuss handling procedures for bilirubin samples.Area of no growth around an antibiotic disk indicates_________________.
- Explain the activities associated with the isolation of Bacillus subtilis organsim from mixed culture to pure culture. Include the relevant information( type of inoculation, source of bacteria etc)? Also the isolation of the organism from the broth containing a fresh mixture of 2 or more bacteria was obtained after incubation of nutreint agar plate.Microbiological Examination of Non-sterile Products 1. The outer surface of the product container was not disinfected. Effect: Rationale:A swab for culture is received from a doctor's office. The staff forgot to mention thesource from where the swab was taken and the climc was closed for next few days.Whatdifferent selective/differential media may be helpful to you to identify the organism.Explain very briefly your rationale for using the particular culture media.