Chemistry 5’G-G-C-T-A-T-T-G-A-G•G-A-T-C-C-T-G-G-A-T-G-C-C3’ 3’C-C-G-A-T-A-A-C-T-C-C-T-A-G•G-A-C-C-T-A-C-G-G5’ a. Give the two DNA fragments generated by the enzyme, and then calculate the binding free energy for re-association of the two, resulting fragments.
Chemistry 5’G-G-C-T-A-T-T-G-A-G•G-A-T-C-C-T-G-G-A-T-G-C-C3’ 3’C-C-G-A-T-A-A-C-T-C-C-T-A-G•G-A-C-C-T-A-C-G-G5’ a. Give the two DNA fragments generated by the enzyme, and then calculate the binding free energy for re-association of the two, resulting fragments.
World of Chemistry, 3rd edition
3rd Edition
ISBN:9781133109655
Author:Steven S. Zumdahl, Susan L. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan L. Zumdahl, Donald J. DeCoste
Chapter10: Energy
Section: Chapter Questions
Problem 2STP
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Chemistry
5’G-G-C-T-A-T-T-G-A-G•G-A-T-C-C-T-G-G-A-T-G-C-C3’
3’C-C-G-A-T-A-A-C-T-C-C-T-A-G•G-A-C-C-T-A-C-G-G5’
a. Give the two DNA fragments generated by the enzyme, and then calculate the binding free energy for re-association of the two, resulting fragments.
b. Calculate the equilibrium constant for the formation of the uncleaved complex at 25°C in 1M NaCl.
c. Calculate the equilibrium constant for association of the ‘sticky ended DNA’ fragments at 25°C in 1M NaCl.
d. Determine the concentration of ds DNA (in part c) after enzyme digestion, if the starting dsDNA concentration was 10-8M.
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