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Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Calculated Numeric Question:
A sample of DNA isolated from a new bacterium contains 34.3% of guanine bases. What percentage of thymine bases would you expect to find in the same sample of DNA?
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Question 8. You isolate the DNA from the bacterial cells and apply the Sanger dideoxy sequencing method. You then separate the products of the reactions by gel electrophoresis and obtain the following pattern. What is the sequence of the template and the DNA on the gel? ddATP ddTTP ddCTP ddGTP Template V [ Choose 3'-CTAGTCAAGG-5' 5'-CTAGTCAAGG-3' Sequence 3'-GATCAGTTCC-5' 5'-GATCAGTTCC-3' 5'-GGAACTGATC-3'
You have a 20 mg/ml of Ethidium bromide stock solution. You need to a final concentration of 2ug/ml into a Agarose solution to visualize DNA. What is the dilution factor? Give typing answer with explanation and conclusion
The Bacteria Escherichia coli DNA genome has a molecular mass of about 3.1 X 109 In your answers, show how you came up to each result? How long is this DNA in micrometer (μm)
A 10 μL-aliquot of a resuspended genomic DNA stock solution was obtained and further diluted by adding 990 μL TE buffer. The A260 of the resulting solution was 0.316. Determine its concentration in µg/µL.
True or False, if the statement is false, provide the correct answer. 1. The amino acid sequence is not responsible for specifying the 3D shape of a protein. 2. The red, orange, and yellow pigments found in plants, that are often visible in the fall are chlorophyll. 3. There are 10 base pairs in one full turn of the DNA double helix. 4. The stroma is a gel-like material within the chloroplast that corresponds to the cytoplasm of the original bacterium. 5. Plants gather the sun's energy with light-absorbing molecules called pigments.
Both protein and DNA are run together in an isoelectric focusing (IEF) electrophoresis using the immobilised pH gradient (IPG) strip with pH range of 4-7. After the electrophoresis and staining, only ONE band is observed on the middle of the IPG strip. The band is a protein band. Briefly explain why only the protein band and NOT the DNA band appear on the IPG strip.
In a sample solution given to be analyzed;CATAGCTTTGTTAAA (DNA nucleotide chain). Show the 5 'and 3' ends by writing in triplet (codon) form. Find the number of Hydrogen bonds in the double helix DNA strand at the beginning.
Instructions In this image, the DNA fragments traveled from top to bottom. Use the drawpad to indicate the positive (+) and negative (-) ends of the gel. Remember, DNA is negatively- charged. Use two colors to circle the shortest fragment and the longest fragment in each of the five samples. 1 2 3 4 5 ||
Analysis of two loci for investigation Someone broke into the bookstore and stole thousands of dollars’ worth of textbooks. Some hairs were left behind, from which DNA was extracted. An individual was caught with all five of the stolen textbooks in his bag. He claims his friend gave them to him. You decide to amplify two regions of the DNA (two loci, plural of locus) and digest the samples, and get the following result on the electrophoresis gel.L = Standard marker 1 = Suspect 1 2 = Suspect 2 3 = DNA from crime scene (Note: assume bands that are close in distance traveled are the same length. Higher concentrations of DNA can make a band appear to travel farther.) What does locus B tell you about the reliability of using loci for analysis?
The chromosome of the bacterium (prokaryote) Escherichia coli has a size of approximately 4,000 kilobases (kb) [one kilobase = 1,000 bases]. What length of DNA (B form) would be contained in it? How does this length compare to the average size and/or volume of this bacterium?
Considering the number of base pairs, compute for the actual length (in micrometers) of this DNA model
Question 4. What is the probability that a restriction enzyme will cut DNA if the recognition sequence for the enzyme is 5'-GGATCC-3'? a) Assuming that the four bases are equally likely to be found in a DNA molecule. O (1/4)6 O (1/4)4 O (1/6)4 O 1 in 6 O (1/6)6

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