Calculate the percentage efficiency of extraction of phycocyanin in sample 3, if the amount of phycocyanin is 59.4 mg, and the starting amount in sample 1 was 98.9 mg.
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Calculate the percentage efficiency of extraction of phycocyanin in sample 3, if the amount of phycocyanin is 59.4 mg, and the starting amount in sample 1 was 98.9 mg.
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- 19) What species if added will allow the separation of neutral molecules in capillary electrophoresis? a) Polyacrylamide b) polystyrene c) sodium dodecyl sulfate d) didodecyldimethylammonium bromide e) divinylbenzeneYou are setting up an experiment to stain cells with DAPI (4',6-diamidino-2- phenylindole). Your stock solution of DAPI is 1 mg/mL. You need your final staining solution to be 1 ug/mL. a) What is the dilution factor you need to use to go from your stock solution (1 mg/mL) to your final staining solution (1 ug/mL)? you can. Show your workings if . b) To what regions of DNA does DAPI bind? Describe in terms of the bases and part of the DNA double helix. c) You notice multiple especially dark speckles in the nuclei of your cells when you look under a microscope. What kind of DNA/chromatin do you think this is? Think in terms of more or less actively transcribed DNA regions.Which is the more effective extraction technique, single or multiple extractions? Explain.
- What does it mean if the color of the solution in UV flashlight are the following Sample 1- light green Sample 2-light green Sample 3- sky blue Sample 4-- blue green Sample 5--cyan blue green Sample 6-- light greenN-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic A purified protein is in a Hepes acid) buffer at pH 7 with 375 mM NaCl. A dialysis membrane tube holds a 2.0 mL sample of the protein solution. The sample tube floats in a beaker containing 1.00 L of the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na+, Cl, and Hepes) can to diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis. Calculate the final concentration of NaCl in the protein sample once the dialysis has come to equilibrium. Calculate the final NaCl concentration in the 2.0 mL protein sample after dialysis in 150 mL of the same Hepes buffer, with 0 mM NaCl, twice in succession. [NaC1] after a single dialysis: [NaCl] after a double dialysis: x10' TOOLS mM mMWhat is the purpose of adding Na2CO3 into the collected tea extract beforeextraction with CH2Cl2?
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