You are setting up an experiment to stain cells with DAPI (4',6-diamidino-2-phenylindole). Your stock solution of DAPI is 1 mg/mL. You need your finalstaining solution to be 1 ug/mL.a) What is the dilution factor you need to use to go from your stock solution (1mg/mL) to your final staining solution (1 ug/mL)?you can.Show your workings if.b) To what regions of DNA does DAPI bind? Describe in terms of the bases andpart of the DNA double helix.c) You notice multiple especially dark speckles in the nuclei of your cells whenyou look under a microscope. What kind of DNA/chromatin do you think this is?Think in terms of more or less actively transcribed DNA regions.

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You are setting up an experiment to stain cells with DAPI (4',6-diamidino-2- phenylindole). Your stock solution of DAPI is 1 mg/mL. You need your final staining solution to be 1 ug/mL. a) What is the dilution factor you need to use to go from your stock solution (1 mg/mL) to your final staining solution (1 ug/mL)? Show your workings if you can. b) To what regions of DNA does DAPI bind? Describe in terms of the bases and part of the DNA double helix. c) You notice multiple especially dark speckles in the nuclei of your cells when you look under a microscope. What kind of DNA/chromatin do you think this is? Think in terms of more or less actively transcribed DNA regions.

You are setting up an experiment to stain cells with DAPI (4',6-diamidino-2- phenylindole). Your stock solution of DAPI is 1 mg/mL. You need your final staining solution to be 1 ug/mL. a) What is the dilution factor you need to use to go from your stock solution (1 mg/mL) to your final staining solution (1 ug/mL)? Show your workings if you can. b) To what regions of DNA does DAPI bind? Describe in terms of the bases and part of the DNA double helix. c) You notice multiple especially dark speckles in the nuclei of your cells when you look under a microscope. What kind of DNA/chromatin do you think this is? Think in terms of more or less actively transcribed DNA regions.

Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter19: Carboxylic Anhydrides, Esters, And Amides

Section: Chapter Questions

Problem 19.34P: 4 (Chemical Connections 19F) Why do Lactomer stitches dissolve within 2 to 3 weeks following...

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