c) As what molecule does each of the monosaccharides enter glycolysis? d) How many moles of ATP are generated from the complete oxidation of one mole of lactose? Show all the steps for the calculation of moles of ATP. Be sure to account for energy used in activating the components of lactose so that they can enter glycolysis.
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- Draw OUT THE arrow pushing reaction mechanism for the following steps of glycolysis: SHOW/IDENTIFY the arrow pushing mechanism in at least 2 steps. CH,OPO CH,OH hexokinase 2+ H H H H H H ОН + ATP OH H ADP + H+ H ОН + НО HO OH H ОН H ОН Glucose-6-phosphate (G6P) Glucosed) How many moles of ATP are generated from the complete oxidation of one mole of lactose? Show all the steps for the calculation of moles of ATP. Be sure to account for energy used in activating the components of lactose so that they can enter glycolysis.Years ago there was interest in using uncouplers such as dinitrophenol as weight control agents. Presumably, fat could be oxidized without concomi- tant ATP synthesis for re-formation of fat or carbohydrate. Why was this a bad (i.e., fatal) idea?
- In the first stage of glycolysis, fructose-1,6-bisphosphate iscleaved to form glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. The latter molecule can then be converted to glyceraldehyde-3-phosphate. Illustrate themechanisms whereby these reactions occurBecause of the position of arsenic in the periodic table, arsenate (AsO43- ) is chemically similar to inorganic phosphate and is used by phosphaterequiring enzymes as an alternative substrate. Organic arsenates are quite unstable, however, and spontaneously hydrolyze. Arsenate is known to inhibit ATP production in glycolysis. Identify the target enzyme, and explain the mechanism of inhibition.The glucose/glucose-6-phosphate substrate cycle involves distinct reactions of glycolysis and gluconeogenesis that interconvert these two metabolites. Assume that under physiological conditions, [ATP] = [ADP]; [P;] = 1 mM. Consider the glycolytic reaction catalyzed by hexokinase: ATP + glucose ADP + glucose-6-phosphate AG = - 16.7 kJ/mol (a) Calculate the equilibrium constant (K) for this reaction at 298°K, and from that, calculate the maximum [glucose-6-phosphate]/ Iglucose] ratio that would exist under conditions where the reaction is still thermodynamically favorable. (b) Reversal of this interconversion in gluconeogenesis is catalyzed by glucose-6-phosphatase: glucose-6-phosphate + H20 = glucose + P AG" = -13.8 kJ/mol
- Glucose is converted to pyruvate in glycolysis, yielding a netsynthesis of 2 ATP. In certain cells pyruvate can be reconverted to glucose during gluconeogenesis. How many ATPsare required to convert pyruvate back to glucose?hexokinase catalyzes the first reaction of the glycolysis pathway. What may happen if there is xylose in the reaction place? Briefly explain.Upon digestion of starch, isomaltose (an isomer of maltose), one of its degradation products, is further hydrolyzed into its monosaccharide components prior to intestinal absorption and entry into the glycolysis. Calculate the number of ATP molecules produced from the digestion and complete oxidation of 1 molecule of isomaltose considering the malate-asparate shuttle. Answer the following items using numerical value only (e.g. 1, not "1 ATP") which will help you arrive at the final answer for this question.
- Relate the names and function and functions of the following glycolytic enzymes to steps in the process of glycolysis 1. Phosphofructokinase 2. Phosphoglyceromutase 3. Triosephosphate isomerase 4. Enolase 5. HexokinaseIf radio-labeled C-2 of glucose is used for glycolysis and alcoholic fermentation, which carbon in the final product(s) is labeled? Please write all steps of glycolysis and alcoholic fermentation, and explain the trace of the radio-labeled carbon in all the key steps (i.e. which carbon is labeled in FBP, DHAP/GAP, and pyruvate).What glycolytic intermediate does glycogenolysis produce? Explain in brief..