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Why is the looping out method preferable for sputum specimen for acid fast smears?
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- What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?How do eosin-methylene blue (EMB) agar plates work? What organism(s) are they designed to detect? How would a positive test appear on the plate?Why is it best to use sterile distilled water in the preparation of microbial suspension and dry smears for slide preparation? What possible error could happen if a glass slide is reused for slide preparation?
- Why are thick or dense smears less likely to provide a good smear preparation for microscopic evaluation?What is the best streaking technique in a culture media? ExplainIn the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?
- What is the principle involved in mucic acid test?Give the procedure of Sabin-Feldman dye test. Describe the positive and negative results.In the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was 7.4 a) Why was the pH of the coating buffer so different?b) What is “coating buffer” for an ELISA? Does the buffer molecule usedmake sense based on the desired pH?In an ELISA procedure the samples are incubated and the ELISA plate iscovered with parafilm and placed in a humidified chamber to preventevaporation of the small liquid volumes in the wells.c) How would your results change if you did not incubate in a humidifiedchamber?
- Why is it necessary to prepare both NSS and Iodine Smears?What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate the pathogen from the sample and why?