Below is the result of PCR products running on agarose gel electrophoresis. You expect to see one band, but the results show two bands, as shown below. Why is there a lower band? What are they? How can we avoid such a band?

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Below is the result of PCR products running on agarose gel electrophoresis. You expect to see one band, but the results show two bands, as shown below. Why is there a lower band? What are they? How can we avoid such a band?

 

 

 

DNA
Standards
A
PCR cycle # 30 35 40
Expected
band
Why is there a lower band
in here? What are they?
Transcribed Image Text:DNA Standards A PCR cycle # 30 35 40 Expected band Why is there a lower band in here? What are they?
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