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- INSTRUCTION: Give the principle or significance of the following practices: NOTE: *please add references* 1. Flaming the mouth or lid of culture tubes or plates after opening and before closing them. 2. Flaming the inoculating needle or loop before and after inoculation. 3. Holding caps, lids, or cotton plugs rather than putting them on the table while inoculating.Instruction: answers must be in numbers. You are culturing bacteria using a petri dish, the bacteria grow very well on this plate you have. However, your microbiology instructor wants to know how many bacteria per milliliter (bacteria/mL) are on your plate. And as you have remembered during the first day of inoculating this bacteria you used a imL aliquots sample of a 1:10,000 dilution, and the total colony you counted on this plate is 170. How many bacterial per milliliters would that be?Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 1. Microscope 2. Colony Counter 3. Autoclave 4. Microbiology incubator 5. Drying oven 6. Refrigerator (microbiology) 7. Bunsen burner/alcohol lamp 8. Candle jar 9. Anaerobic jar 10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet 11. Bacteriologic filters (Seitz, Chamberlain, Berkfield) 12. Petri dish 13. Culture tubes 14. Hanging drop slide 15. Durham’s tube 16. Staining rack 17. Thermostatically controlled water bath 18. Inoculating loop 19. Inoculating needle 20. Vials
- Questions: 1. What happens to the number of bacteria as you streak from one sector to another? 2. What happens to the colonies when the bacteria are separated well? 3. How bacteria does each colony come from? many 4. Why do you make sure that the inoculating loop is red hot? 5. What happens when the streaking is not correct? 6. When do you flame the mouth of the cultures tubes? 7. Why you should not use the inoculating loop when it is red hot?Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 1. Microscope 2. Colony Counter 3. Autoclave 4. Microbiology incubator 5. Drying oven 6. Refrigerator (microbiology) 7. Bunsen burner/alcohol lamp 8. Candle jar 9. Anaerobic jar 10. Microhood or Bacteriologic hood/Safety hood/Safety cabinetwebapps/assessment/take/launch.jsp?course_assessment_id%3D 498749 1&course_id3 111786 1&content id=D Customize Links Free Hotmail Windows Windows Media Imported From IE Importe Serial dilution QUESTION 2 What type of agar is TSA? (select all that apply) Nutrient
- Guide Questions: Differentiate the types of culture media based on its physical state and uses. What makes agar good support for microbial growth? Define and contrast the terms “sterile” and “clean”. List 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?困 Topic: laboratory Instrument Which of the following are TRUE regarding Which of the following is/are TRUE the use of laboratory instruments and regarding the use laboratory instruments equipment in regards to making of culture media? " and equipment? * Air bubbles on the surface of agar plates Hot plates can be used to sterilize the inoculating loop and needle. can be removed by fanning them with the luminous flame of the bunsen burner. If the agar surface is wet, it can be dried by A drying oven is used to sterilize nutrient agar, a solid culture medium. heating at 30-40 deg C in the drying oven before inoculation is done. The refrigerator is used to grow psychrophiles. All media taken from the refrigerator should be warmed to room temperature before use, P Type here to search 7:11 pm 30/09/2021 ヘロG) ENG 近Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 6. Refrigerator (microbiology) 7. Bunsen burner/alcohol lamp 8. Candle jar 9. Anaerobic jar 10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet 11. Bacteriologic filters (Seitz, Chamberlain, Berkfield) 12. Petri dish 13. Culture tubes 14. Hanging drop slide 15. Durham’s tube
- :Objectives on Agar plates and line Streaking method-13 Two students applied following are the results they obtained :Student A Student B Who PSucceeded PWho failed? WhyGive the uses/functions and images of each apparatuses Basic Laboratory Equipment Uses/Functions Picture 21. Antibiotic disk 22. Antibiotic dispenser 23. Weighing balance 24. Forceps 25. Stains/Staining reagents 26. Culture media/Culture plates 27. Culture swabs 28. Gloves 29. Mask 30. Caliper rulerProcedures: 1. Place one piece of fruit in a Ziploc bag. 2. Smashgrind up the fruit using your fist for 2 minutes. Careful not to break the bag! 3. Measure 10ml of extraction buffer (soap and salt solution) and add it to the bag. 4. Kneed/mush the fruit in the bag again for 1 minute. 5. Assemble our filtration apparatus. Place your test tube/glass into a rack to keep it stable. One person places the mesh over the funnel and holds it tightly as the other members pour the fruit slurry into the filtration apparatus, draining only the liquid into the test tube/glass, Discard left-over chunks of fruit on the mesh, 6. Slowly pour cold rubbing alcohol into the tube/glass, being careful to tit the test tube/glass to allow the rubbing alcohol gently pour into the test tube/glass fruit liquid. Do not quickly dump straight into the fruit liquid. OBSERVE 7. Dip the loop or glass rod into the test tubeiglass where strawberry extract and rubbing alcohol layers come into contact with each other.…