A solution containing 3.51 mM X (analyte) and 1.67 mM S (internal standard) gave peak areas of 3505 and 10437, respectively, in a chromatographic analysis. Then 1.00 mL of 8.47 mM S was added to 5.00 mL of unknown X, and the mixture was diluted to 10.0 mL. This solution gave peak areas of 5428 and 4431 for X and S, respectively. Answer the following questions and enter your results with numerical values only. Calculate the response factor for the analyte. (keep four significant figures) Find the concentration (mM) of X in the 16.0 mL of mixed solution. Find the concentration (mM) of X in the original unknown.

A solution containing 3.51 mM X (analyte) and 1.67 mM S (internal standard) gave peak areas of 3505 and 10437, respectively, in a chromatographic analysis. Then 1.00 mL of 8.47 mM S was added to 5.00 mL of unknown X, and the mixture was diluted to 10.0 mL. This solution gave peak areas of 5428 and 4431 for X and S, respectively. Answer the following questions and enter your results with numerical values only. Calculate the response factor for the analyte. (keep four significant figures) Find the concentration (mM) of X in the 16.0 mL of mixed solution. Find the concentration (mM) of X in the original unknown.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter26: An Introduction To Chromatographic Separations

Section: Chapter Questions

Problem 26.14QAP

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