6. Discuss the potential contributions of ionic interaction, hydrogen bonds, hydrophobic interaction, and van der Waals interactions for the side chains of Asp, Leu, Tyr, and His in a protein.
Q: what is The standard free energy change for the combined system that you wrote in the previous…
A: The standard free energy change (∆G°) of a chemical reaction is the free energy change at the…
Q: A) H H₂N-C-C <=0 CH2 OH ОН н D) H2N-C-C <=0 CH2 OH HO CH3 B) H₂N-C H3C-CH CH2 CH3 0= OH E) H2N-c-c С…
A: Amino acids are simply an alpha-carbon bonded to 4 different groups. The 4 groups are;an alpha-amino…
Q: Create a figure that includes a schematic of the topological map of AlaE. In your topological map,…
A: Topology data from 3 different techniques; hydropathy profile analysis, TMHMM and TOPCONS are…
Q: An engineered ligand binds its target with 5 nM affinity at high pH. However, protonation of a…
A: An engineered ligand (L) binds to a target protein (T) to form a ligand:target complex (LT). The…
Q: 1. Shown below is the intermediate in the aldolase mechanism, after dissociation of the first…
A: When drawing chemical reaction mechanisms, we use a curved arrow to show the flow of electrons. The…
Q: The protein on the right called desmolaris, also called Draculin, found in bats. It is a non…
A: An injury to blood vessels leads to the leakage of blood and this is stopped via a process called…
Q: addition to L-lactate, which of the following molecules is (are) generated by fermentation of…
A: Fermentation is an anaerobic process that occurs in the absence of oxygen, leading to the conversion…
Q: Make a figure that includes the hydropathy profile analysis, the TMHMM analysis and the TOPCONS…
A: The 3-D structure (topology) of transmembrane proteins can be studied with relative accuracy using…
Q: pindle The cell above is in a) Meiosis 2 b) Meiosis 1 c) Mitosis ****
A: Given cell is in metaphase stage. It is a mitosis cell division.
Q: In experiments carried out in 1941 to investigate the citric acid cycle, oxaloacetate labeled with…
A: The given reactions are part of a metabolic cycle known as the citric acid cycle or Krebs cycle. The…
Q: 8. Amino acids typically consist of a central carbon atom. Attached to this central carbon are an…
A: If the a-hydrogens in the a-alpha helix are replaced by a second side chain (a second R group), it…
Q: Question 27 These are all examples of polysaccharides except_ a. starch b. glucose c. cellulose d.…
A: Starch: Starch is a polysaccharide that serves as a storage form of energy in plants. It consists of…
Q: Why do you see a sharp decline in this graph at pHs higher and lower than 6? Effect of pH on Enzyme…
A: The graph shows how pH affects the activity of the enzymes. Enzyme activity is displayed on the…
Q: Genetics Question 12
A: The objective of the question is to identify the stage of cell division during which crossing over…
Q: Please fill in the four boxes bolded below you KM (MM) Vmax (nM/s) Type of inhibition…
A: Competitive inhibitors are the substances which are similar to substrate molecules in their…
Q: 11. A polypeptide is making a short a-helix. A typical residue in an a-helix is involved in two…
A: In an alpha helix, each amino acid residue forms hydrogen bonds with the amino acid located three…
Q: Which of the following are attributes of a well-prepared graph? (Select all that apply.) O labels on…
A: In a laboratory manual, a graph typically refers to a visual representation of data collected during…
Q: 6. Use the density of water to determine the volume of water delivered. 7. Calculate the mean of…
A: In the given part 6to calculate the volume of water deliveredformula used= Density=…
Q: Sickling occurs in deoxyhemoglobin S but not in oxyhemoglobin S. Oxyhemoglobin has a small,…
A: Sickling is the process where the haemoglobin protein inside the RBC cell clumps together this…
Q: In a uni uni enzyme reaction, what is the substrate concentration relative to Km when an enzyme…
A: For a one-substrate enzyme-catalyzed reaction, the Michaelis-Menton equation shows the quantitative…
Q: The bacterial gene shorty (sh) encodes for a small protein. The DNA sequence of the sh gene is shown…
A: The objective of the question is to determine the length of the Sh protein in amino acids (AA's)…
Q: In a reaction system, the concentrations of Enzyme-Substrate complex [ES], free enzyme [E] and free…
A: For a reversible reaction, the ratio of the product concentration to the reactant concentration at…
Q: Luther found a vial containing an unknown lipid sample. He separated the different lipids using gel…
A: The dimensionless number is used in TLC to measure a substance's relative movement concerning the…
Q: Why can’t we see the double helix structure of DNA of strawberry and kiwi?
A: The question is asking why we can't visually observe the double helix structure of DNA in…
Q: 2. Shown below is the enol intermediate in the citrate synthase mechanism. Draw the carbanion…
A: Citrate synthase is an enzyme in the citric acid cycle. It catalyses the condensation of acetyl CoA…
Q: Part D Tubes 1 weight w/o water graph 25 3 4 5 61718 9 1.065g 1.073g 1.072 1.082 1.090g|1.105 g.…
A: Pipetting is a fundamental technique in biochemistry used for the accurate and precise transfer of…
Q: 2B. S. aureus hemolysin B attacks the RBC cell membrane by hydrolyzing the sphingomyelin headgroup:…
A: Sphingomyelin is a phospholipid which has phosphocholine as head group and sphingosine as platform…
Q: Draw the following amino acids linked by peptide bonds: a. aspartate b. lysine c. cysteine d.…
A: Amino acids are compounds containing carbon, hydrogen, oxygen and nitrogen. They are monomers or…
Q: Imagine a typical enzyme kinetic curve of [S] vs. V. The maximum [S] is about four times the Km.…
A: 1. Vmax: A parameter in enzyme kinetics, maximum velocity, or Vmax for short, is the fastest rate at…
Q: Genetics Question 20
A: The objective of this question is to determine the correct order of genes on a chromosome map based…
Q: Question 2 Listen The ABO gene is on a different chromosome from the +/- (Rh factor) gene. What…
A: The objective of the question is to determine the correct genetic designation for a person with O…
Q: When the CAC is run in reverse by microorganisms that use it to fix carbon, the citrate synthase…
A: Kreb cycle is the reaction procews that operates in the mitochondria and it is followed by…
Q: Dihybrid Cross 13 Problem 5: A SsYy x ssyy test cross. Which of the following genotypes would you…
A: The objective of this question is to determine which genotype would not be expected in the offspring…
Q: Which of these situations would produce a Hill plot with a Hill coefficient less than 1? A purified…
A: A Hill plot is used to establish the cooperativity of ligand binding to a protein.NH (Hill…
Q: The image below is from what stage a) Metaphase b) Interphase c) Telophase d) Anaphase e) Prophase
A: Cell division typically involves two main stages: mitosis and cytokinesis. Here's a brief overview…
Q: 7) The following questions apply to glycolysis (figure 3-41 on page 79 of the 12th edition), the…
A: Note : Please note that according to guidelines only first 3 subparts should be solved. Please…
Q: Problem 2
A: The objective of this question is to identify the recombinant offspring from a genetic cross between…
Q: You have an aqueous solution of hemoglobin (pl = 6.8) at pH 6.5, and currently the proteins are…
A: pH affects the ionisation of amino acids and can thus influence the nature and distribution of…
Q: Genetics Question 9
A: The objective of the question is to identify the event that occurs last during meiosis and the…
Q: A portion of the sequence from the DNA coding strand of the chick ovalbumin gene is shown. Determine…
A: Transcription is the process during which the DNA double helix unwinds and the RNA polymerase enzyme…
Q: O Steps of Translation 1. 2. The ribosome looks for leaves the nucleus and binds to a Codon: group…
A: Translation is generally defined as a process in the living cells in which the proteins are…
Q: 22. For the process, A B, Keq is 0.02 at 37°C. For the process, BC, Keq 1000 at 37°C. A. Determine…
A: The objective of the question is to determine the equilibrium constant for the overall process A…
Q: Your friend hasn't been feeling well, and you think that she should get tested because it could be a…
A: PCR or polymerase chain reaction is a lab procedure that amplifies fragments of DNA or RNA by using…
Q: Enzymes are biological catalysts that decrease the activation energy necessary for a chemical…
A: Oxidoreductases are a class of enzymes that catalyze redox reactions. Oxidoreductases generally uses…
Q: . If 20 mM solution of Acetyl-phosphate is transformed to acetate by incubating with a catalytic…
A: Initially there was 20 mM acetyl-phosphate. At equilibrium, the acetyl-phosphate concentration…
Q: Explain in why carbohydrates can be bad for you( give 3 examples)
A: Carbohydrates — fiber, starches, and sugars — are essential food nutrients that your body turns into…
Q: Molecule A held promise as a treatment for rheumatoid arthritis except that it remained in the body…
A: Many factors determine the duration of time a drug stays in the body. The drug being hydrophobic…
Q: Cellulose contains___ linkages that ___ be digested by animals. a β1--> 4 glycosidic ; canNOT b…
A: Cellulose is a polymer of units. It is used by plants and some microbes as a means of mechanical…
Q: A potent inhibitor effectively inhibits an enzyme catalyzed reaction. What kind of a Ki value you…
A: An inhibitor is defined as a molecule that binds to an enzyme and decreases its activity whereas a…
Q: 6. Consider the heptapeptide DERHHKY. What is the overall charge of this peptide at pH 5 and pH 7.4?…
A: Amino acids are organic molecules that form the building blocks of proteins. They contain an amino…
Step by step
Solved in 1 steps
- 1.Here is an oligomeric protein, which has two binding sites:1)Write the formulas of side chains of amino acids which are located in binding sites and suggest two ligands,which can be bound with this protein. 2) Name the types of bonds which will be formed between the ligands and amino acids of binding sites.3)Give the definition of quaternary structure. What do you know about the properties of proteins with the quaternary structure?1. The following protein crystal structure is the Human Serum Albumin complexed with octadecanoic acid (PDB ID: 1E7I). According to the protein data bank, Human Serum Albumin is composed of 72% helical structure. Among the following options (a-c), select the possible Ramachandran plot for the above Human Serum Albumin. (a) (b) (c) Phi Phi Phi1.Describe in detail how to determine the primary structure of protein. 2.You have been given a mixture of lysine, histidine and cysteine.The isoelectric point of the amino acids are as follows; histidine 7.64 lysine:9.74 cysteine:5.02 Show how you will separate the mixture into the pure forms. State and describe any instrument that you will use to separate the components in the mixture.
- 1.Describe in detail how to detect the primary structure of protein. 2.Given a mixture of lysine,histidine and cysteine.The isoelectronic point of the amino acids are as follows: histidine:7.64 lysine:9.74 cysteine:5.02 Show how you will separate the mixture into the pure forms. State and describe any instrument that you will use to separate the components in the mixture.11. Below is a folding energy funnel describing folding energy landscape of a protein. The width of the funnel indicates the entropy of the protein, and the height corresponds to the free energy. A) If A is the native fold structure, which state is a molten globule? How does this state differ from A in term of structures. B) Does this protein have multiple folding pathways or just one? C) which state has the lowest free energy? D) According to the width of the funnel, the native state B of the protein has the lowest entropy. If the protein fold A spontaneously to this state, does it violate the 2nd law of thermodynamics? Why or why not? (Hint: in the folding funnel, only the entropy of the protein alone is considered). E) Does the native state also have the lowest enthalpy. What makes the enthalpy decrease as the protein folds? 12. List four methods by which a protein can be denatured and briefly describe how these methods act to disrupt protein structure.5. Indicate whether the following amino acid residues would be more likely to be found on the surface or in the interior of a folded protein: Leu, Arg, Phe, Asn, and Glu. Give your reasoning for each case (diagrams not required).
- 24. What is the quaternary structure of a protein? Do all proteins have quaternary structure?2. a. In the following diagram of a portion of a protein, label the types of interactions that are shown. b. What level of protein structure are these interactions producing? b. CH CH3 CH3 Polypeptide backbone CH2 H3C H3Ç 10 CH С—ОН - CH,-S-S-CH,- а. CH2 с. -CH,-CH,-CH2-CH2-NH 0-C-CH2- d.7. Identify the primary structure of a hexapeptide containing five different amino acids if the following smaller peptides are among the partial hydrolysis products: Gly-Cys, Ala-Ser, Ala-Gly, and Cys-Val-Ala
- 2. A functional protein that is approximately 110 kDa in size has all its cysteine residues joined with disulfide bonds. It has one cysteine residue per 100 amino acids. When heated at 85°C it lost activity, but when it was allowed to cool, the activity was restored? Please answer the following questions: a) How many disulfide bonds the protein has? Show how you came up with the answer. b) What is the molecular basis for the protein behavior in restoring activity?1. An intrinsically disordered protein (IDP) is a protein that lacks a fixed three- dimensional structure. Estimate the size of an IDP of 100 amino acids by calculating the most probable end-to-end length of the protein. Assume the protein is a freely-joined chain with the length of each segment (amino acid) b = 3Å.1)Explain 3 benefits of proteins forming higher oligomeric states. 2) Why are peptide bonds planar?