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- 1- Why was the T4 and E.coli mixed together in top agar and then poured on the plate instead of just spreading the two together with a glass spreader on the plate? 2- When doing the titration and isolation of the T4 phage, E.coli diluted for you to 10e7 cells/ml. why was a diluted culture used? 3- After doing the titration of T4 phage, you find your dilution plates are all TNTC. how could this have happened? 4- Could you isolate phages that infect E.coli from raw sewerage? explain.1) would you describe the contents of the soil-inoculated broth as being a “pure culture”? Why or why not? 2) How did the uninoculated broth differ in appearance from the broths inoculated with E. Coli and M. Luteus? And then how could you tell if a supposedly sterile, uninoculated broth was contaminated? Please explain in detail and highlight the important parts cuz I am confused and need help! Thanks1. You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, you are observing the growth of the plate prepared from the 10-8 plate. You divide the plate into two equal parts and count 46 colonies in each half.a) What was the dilution factor?b) How many total colonies were on the plate?c) Was your count valid?d) How many bacteria were present in the soil sample?
- 1. The colonies on a negative MSA plate would appear _____________. 2. "E. Coli and S. epidermidis were chosen to represent Gram-negative and Gram-positive bacteria, respectively. For a given antibiotic, is there a difference in susceptibility between the Gram-positive and Gram-negative bacteria?" "No, they are usually similar" "yes, drugs that target the cell membrane are more effective on gram negatives" "yes, drugs that target the cell wall are more effective on gram negatives" "yes, drugs that target the cell wall are more effective on gram positives" how can I tell the difference8. Pigment or color (e.g.: opaque, translucid, red, yellow, rose, violet, etc..) is also another characteristic that can be used to identify your microorganism in a slant Thus, write down the name of three microorganisms (using scientific notation: Escherichia coli) that present a pigmented pattern of growth in a slant (write down the color of each pigment) and the name of the disease that they may cause. F10 F12 F6 %24 6. R. Home Enter K F G A Shift C Page Up Page Down Alt Ctrl Alt Σ8. Pigment or color (e.g.: opaque, translucid, red, yellow, rose, violet, etc..) is also another characteristic that can be used to identify your microorganism in a slant. Thus, write down the name of three microorganisms (using scientific notation: Escherichia coli) that present a pigmented pattern of growth in a slant (write down the color of each pigment) and the name of the disease that they may cause. 200%- 927 PM 0 日 2/7/2021 21 DELL Insert Delete F11 F12 PrtScr F9 F10 F7 F8 F6 F3 F4 F5 Esc F1 F2 Backspace & # 2$ 8 4 1 2 P Home R T E Enter K Shift V B Page Down Page Up Alt Ctrl Alt THome ノ/ *00 Σ
- You are working in a lab studying Streptococcus pyogenes as a cause of necrotizing fasciitiis. You have an overnight culture that you want to know the starting concentration of, so do a set of six 1:10 serial dilutions (putting 1 mL from the stock into a 9 mL blank), with tube #1 being 1:10, #2 is 1:100, etc. You plate 0.1 mL from tube 5 onto a blood agar plate and the next morning count 134 colonies. How many bacteria (measured in CFU/mL) were in the overnight culture flask? A. 1.34 x 10^4 CFU/mL B. 1.34 x 10^5 CFU/mL C. 1.34 x 10^6 CFU/mL D. 1.34 x 10^7 CFU/mL E. 1.34 x 10^8 CFU/mL F. cannot tell based on the data given - you'd need to know the volume of the original culture flaskWhen bacteria from a throat swab are streaked on blood agar, why is the agar stabbed several times with the loop?1. What color does an acid-fast cell stain? 2. Identify two diseases that are caused by acid-fast bacteria. 3. In the endospore stain, what color do the endospores stain? 4. If you performed an endospore stain on Mycobacterium, what color cells would you expect tosee? Why? 5. How do capsules contribute to virulence of an organism? 6. Since you know what lophotrichous and amphitrichous arrangements look like, put thoseterms together to draw an amphilophotrichous bacterium. 7. Streptococcus pneumoniae that are capable of causing pneumonia are encapsulated bacteria(meaning they have a capsule). Describe what you would expect to see under themicroscope after performing a capsule stain with india ink and safranin.
- You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.a) What was the dilution factor?b) How many bacteria were present in the soil?2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.a) After 5 hours, how many generations have occurredb) After 5 hours, how many bacteria are present?3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?1. What is the most common sterilization technique used in laboratories? 2. List at least 5 procedures of the aseptic technique and describe its uses. 3. Why is Gram stain one of the most important and widely used stains in bacteriology? 4. Explain the Gram staining technique in chronological order. Indicate the reagent used and the time of usage on each reagent.Abigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies but there is a smear over the entire plate. She was working with E. coli. Why does she have a smear?