If a pediatric vitamin contains 1500 units of vitamin A per milliliter of solution, how many units of vitamin A would be administered to a child given 2 drops of a solution from a drpper calibrated to deliver 20 drops per milliar of solution?
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If a pediatric vitamin contains 1500 units of vitamin A per milliliter of solution, how many units of vitamin A would be administered to a child given 2 drops of a solution from a drpper calibrated to deliver 20 drops per milliar of solution?
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- Using the data from the standard curve done previously, you record the absorbance of your extracted milk protein sample at 0.499 at 540nm. What is the mass of the protein in mg assuming you collected 10mL worth of the protein? Your standard curve should have a line of best fit with the equation y=0.0001x+0.0038.A stock solution contains 0.2 mg/mL protein. From this stock solution, 179 uL is diluted with 1.1 mL buffer. What is the concentration of protein in this dilution?If the average molar mass of a sample of soybean oil is 1500 g/mol, how many grams of NaOH are needed to saponify 5.0 g of the oil?
- In order to prepare a standard curve, you will do serial dilutions of the standard protein. Assume the protein concentration in tube A is 3 mg/ml and you take 0.5ml and add it to the next tube (tube B) containing 0.5ml of buffer. What is the concentration in tube B?Answer the following questions about protein concentration determination. a) At what wavelength should an albumin protein solution placed in a quartz cuvette be measured in the spectrophotometer? Why is that? b) With which methods other than spectrophotometer can the amount of protein be measured quantitatively?You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule, and the spectrophotometer reading at 595 nm is 0.6. Using the attached calibration curve(click to enlarge), calculate the concentration of protein in undiluted egg white. Give your answer in mg/ml.
- The iodine contained in every one mL of the 0.010 M iodine solution in the buret will react with 1.76 mg of vitamin C. (In other words, every mL of iodine solution needed for the titration indicates the presence of 1.76 mg of vitamin C in the juice sample.) If your titration used 7.11 mL of iodine solution, what mass of vitamin C is in your fruit juice sample (in milligrams, mg)? Include the unit and two decimal places in your answer.A mixture of five proteins are analyzed by MALDI-TOF. Their molecular weigths are 3 kD, 22 kD, 100 kD, 225 kD and 515 kD. A peak appears in the mass spectrum at 33.3 kD. Which protein most likely gave this peak?An ion-exchange chromatographic separation is performed using a diethyl-aminoethyl- (DEAE)-sepharose column to separate proteins in a mixture. The mixture contains Protein A (pl=6.0), B (pl35.0), C (pl=7.5), D (pl =1), and E (pl=4.0). The protein mixture is prepared in a buffer solution pH =5. When the protein mixture is loaded onto the column, and the column is washed with a buffer solution pH 5, which protein(s) will be captured by DEAE-sepharose in the column? O Protein B because it is predominantly in net negative charge form. O Proteins D and E because they have predominately net negative charge in pH 5 solution O Proteins A, C, D and E because they have charges O Proteins A and C because both both predominantly have net positive charges O Proteins Band E because both predominantly have net positive charges
- You receive an order for “heparin 6,000 units SQ q 12 hrs”. The stock solution contains heparin 5,000 units per 0,5 mL. How many milliliters must be administered?It is desired to measure the acidity values of two solid fats coming to a food analysis laboratory. In this experiment to be carried out with the titration method, 0.1N KOH was used, and two separate titrations were made, one for the old oil and one for the new oil. As a result of the titration, 4.6 mL of KOH was spent for one and 9.1 mL of KOH for the other. If the amount of oil weighed for both is 10 g and the amount of KOH consumed in the oil-free titration is 1.2 mL, calculate the acidity values for both oils, write which value this value belongs to and the reason for it.A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show working