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4 A lab technician has 90 ml of crude extract from a mouse heart. They want to determine how many total I.U.'s of MDH they have in the extract. They take 0.1 ml of the extract and add it to 900 μl of 20 mM MOPS, pH 7.4 (dilution A). After mixing

4 A lab technician has 90 ml of crude extract from a mouse heart. They want to determine how many total I.U.'s of MDH they have in the extract. They take 0.1 ml of the extract and add it to 900 μl of 20 mM MOPS, pH 7.4 (dilution A). After mixing

Curren'S Math For Meds: Dosages & Sol
Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter1: Relative Value, Addition, And Subtraction Of Decimals
Section: Chapter Questions
Problem 39SST
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Question: 4 A lab technician has 90 ml of crude extract from a mouse heart. They want to determine how many total I.U.'s of MDH they have in the extract. They take 0.1 ml of the extract and add it to 900 μl of 20 mM MOPS, pH 7.4 (dilution A). After mixing gently, they take 900 μl of dilution A and add it to 800 μl of 20 mM MOPS, pH 7.4 (dilution B). They then run the assay that is listed below: 900 μl 20 mM MOPS, pH 7.4 10 μl 10 mM NADH (in H2O) 10 μl 25 mM oxaloacetate (in H2O) 30 μl of dilution B of their MDH solution, in 20 mM MOPS, pH 7.4 They mix everything gently together and then measure the AA340/A time. Their measured AA340/20 sec. was "-0.290". How many total I.U.'s of MDH was in the crude extract? PLEASE SHOW EACH STEP MATHEMATICALLY
Transcribed Image Text:Question: 4 A lab technician has 90 ml of crude extract from a mouse heart. They want to determine how many total I.U.'s of MDH they have in the extract. They take 0.1 ml of the extract and add it to 900 μl of 20 mM MOPS, pH 7.4 (dilution A). After mixing gently, they take 900 μl of dilution A and add it to 800 μl of 20 mM MOPS, pH 7.4 (dilution B). They then run the assay that is listed below: 900 μl 20 mM MOPS, pH 7.4 10 μl 10 mM NADH (in H2O) 10 μl 25 mM oxaloacetate (in H2O) 30 μl of dilution B of their MDH solution, in 20 mM MOPS, pH 7.4 They mix everything gently together and then measure the AA340/A time. Their measured AA340/20 sec. was "-0.290". How many total I.U.'s of MDH was in the crude extract? PLEASE SHOW EACH STEP MATHEMATICALLY
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