Effect of Concentration on Enzyme Activity Lab Report

The more acidic a substance is the less oxygen it will produce when going through a chemical reaction. During the Lab “How Do Changes in pH Levels Affect Enzymes Activity”, the researcher conducted an experiment to test the effects that an acidic, neutral, and a base substance will have when combine it with hydrogen peroxide. The data table shows that HCL (acidic substance) barley produced any oxygen at all when it was combining with Hydrogen Peroxide. The pH level for HCL was 2.5; this level indicates that the substance was very acidic. When the H2O and NaOH were tested they produced more bubbles than HCL. NaoH produced a little more bubbles than HCL. The pH that NaoH produced was a 9, which is a base. H2O produced more bubbles than both substances;

This experiment looked at how substrate concentration can affect enzyme activity. In this case the substrate was hydrogen peroxide and the enzyme was catalase. Pieces of meat providing the catalase were added to increasing concentrations of hydrogen peroxide in order to measure the effect of hydrogen peroxide concentrations on the enzyme’s activity. The variable measured was oxygen produced, as water would be too difficult to measure with basic equipment.

Enzymes are biological catalysts, which means it decreases activation energy in reactions. The lower activation energy in a reaction, the faster the reaction rate. Many enzymes alter their shape when they bind to the activation site. This is called induced fit, meaning for the enzyme to work to its full potential it has to change shape to binding substrate. The location of enzyme’s activation site is on the surface of the enzyme, where the binding of substrates take place. Enzyme activity can be influenced by a variety of environmental factors. If the concentration of enzyme is low, and there is a great deal of substrate, then increasing enzyme concentration results in more molecules available to convert substrates to products. Thus, increasing enzyme concentration can increase reaction rate. If substrate concentrations are low, and many of the existing enzymes are idle because of a lack of substrate, then adding enzyme will have no effect on reaction rate. Enzyme concentration affects the enzyme activity, because the more enzyme concentration the faster the reaction rate, until it hits it’s limiting factor. When substrate concentration is increased, it also increases rate of reaction. Temperature plays an important

I would guess that the surface area has been decreased and the diffusion distance for gas

_The tank is compressing the helium into a denser state but when it is put in the balloons it expands and takes up more space. __________________________________________________________________________

Enzymes are types of proteins that work as a substance to help speed up a chemical reaction (Madar & Windelspecht, 104). There are three factors that help enzyme activity increase in speed. The three factors that speed up the activity of enzymes are concentration, an increase in temperature, and a preferred pH environment. Whether or not the reaction continues to move forward is not up to the enzyme, instead the reaction is dependent on a reaction’s free energy. These enzymatic reactions have reactants referred to as substrates. Enzymes do much more than create substrates; enzymes actually work with the substrate in a reaction (Madar &Windelspecht, 106). For reactions in a cell it is

This investigation will be carried out to investigate the rate of reaction of the enzyme catalase on the substrate hydrogen peroxide.

If the substrate concentration is increased then the rate of a catalase reaction will also increase until it reaches the optimal concentration or saturation point and will remain constant. This is because there will be more substrate molecules in a higher concentration therefore a higher frequency of collisions. This increases enzyme activity and more product will be formed. However at a certain concentration the enzymes will become saturated (all

Question: How does changing enzyme concentration or temperature affect the reaction time of enzyme activity?

However, the rate of reaction only increases for a certain period of time until there is lesser substrate molecules than the enzyme molecules. The increase of enzyme concentration does not have effect if there are lesser substrate molecules than enzyme molecules initially.

The concentration of enzymes and substrates affects the rate of reaction, as the concentration of the enzyme and substrate is increased, the velocity of the reaction proportionately increases. By increasing the concentration of substrate it will gradually increase the velocity of enzyme reaction within the restricted range of substrate levels.

The objective of the experiment is to measure and compare the enzyme activity (the enzyme Catalase which is extracted from chicken liver) and the factors that speed up the enzyme action such as concentration of the enzyme, temperature, and pH. This experiment was also used measure the production of oxygen gas using a fermentation vial as it reduces hydrogen peroxide. If the peroxide is destroyed within the experiment, there is a lesser amount available to have a reaction while the oxygen is produced at a lower rate. With that in mind, if there isn't any hydrogen peroxide left; oxygen is no longer created.

Enzymes are substances, most of protein that at as biological catalysts. This means that Enzymes speed up chemical reaction by lowering activation energy by promoting the conversation of reactant in to products. Also enzymes are shape specific, because of the highly specific complementary fit between the three dimensional shape of the enzyme active site. Substrates are the reactant of a reaction that the enzyme able to catalyze the reaction. The react of reaction increased as the increase of amount of enzyme present. Moreover, the rate of reaction will initially increase as the increase of the amount of substrate.to function properly and efficiently, most enzyme

The purpose of this lab report is to investigate the effect of substrate concentration on enzyme activity as tested with the enzyme catalase and the substrate hydrogen peroxide at several concentrations to produce oxygen. It was assumed that an increase in hydrogen peroxide concentration would decrease the amount of time the paper circle with the enzyme catalase present on it, sowing an increase in enzyme activity. Therefore it can be hypothesised that there would be an effect on catalase activity from the increase in hydrogen peroxide concentration measured in time for the paper circle to ride to the top of the solution.

Enzymes are proteins that act as catalysts and help reactions take place. In short, enzymes reduce the energy needed for a reaction to take place, permitting a reaction to take place more easily. Some enzymes are shape specific and reduce the energy for certain reactions. Enzymes have unique folds of the amino acid chain which result in specifically shaped active sites (Frankova Fry 2013). When substrates fit in the active site of an enzyme, then it is able to catalyze the reaction. Enzyme activity is affected by the concentrations of the enzymes and substrate present (Worthington 2010). As the incidence of enzyme increases, the rate of reaction increases. Additionally, as the incidence of substrate increases so does the rate of reaction.