Using retroviral vectors for gene therapy might increase the patient's risk of developing cancer because they might: a. introduce proteins from the virus b. integrate recombinant DNA into the genome in ways that misregulate the expression of genes at or near the site of integration c. not express the genes that were introduced into a patient's cells d. not integrate their recombinant DNA into the patient's genome

Using retroviral vectors for gene therapy might increase the patient's risk of developing cancer because they might: a. introduce proteins from the virus b. integrate recombinant DNA into the genome in ways that misregulate the expression of genes at or near the site of integration c. not express the genes that were introduced into a patient's cells d. not integrate their recombinant DNA into the patient's genome

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 13SQ

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Similar questions

. a. Compare the means by which retroviral and AAVvectors deliver therapeutic genes to human cells.b. Explain the advantages and disadvantages of eachof the two viral vectors.
Define the following terms:a. satellite DNAb. transpositionc. transposond. retrotransposone. endogenous retrovirus
A" is a retrovirus. It can integrate DNA into random sites of human genome. Another virus "B" can integrate DNA at a specific site of human genome. Which one of these viruses would make a better vector for gene therapy? Briefly explain.
Which of the following best describes the approach that wasused in the first gene therapy trial for treating SCID?a. The normal ADA gene was introduced by injecting liposomesdirectly into the patients’ bodies. b. Lymphocytes were removed from a SCID patient, the normalADA gene was transferred into the lymphocytes vialiposomes,and then the lymphocytes were returned to thepatient’s body.c. Lymphocytes were removed from a SCID patient, the normalADA gene was transferred into the lymphocytes via a retrovirus,and then the lymphocytes were returned to thepatient’s body.d. None of the above describe the approach used in the trial.
Which of the following is correct regarding the vector vaccine? Select one: a. It is a vaccine which uses a non-pathogenic virus to introduce pieces of the pathogen to the host b. It is a vaccine which is made by killed pathogen c It is a vaccine which is not alive but uses cloned genes from the pathogen in a vector d. It is a vacciñe which is considered as an attenuated vaccine but carries the pathogen genes in a vector e It doesn't need cloning steps
You are researching how the virus that you are studying spreads through a host. Which of the following assays would be the most advanced to evaluate viral spread in real-time? euthanization of SCID mice followed by dissection and flow cytometry euthanization of mice infected with engineered viruses that express reporter proteins and staining for protein expression euthanization followed by dissection and assaying for the virus in tissues MRI imaging of mice expressing viral constructs that express bioluminescence
if you encounter an unknown disease in humans and would like to understand the genetic mechanism for this disease so that an effective and targetted treatment approach can be developed , what will be the best strategy or steps to accomplish the goal? NB :answer in at most 4 sentences.
Which of the following is a concern of in-vivo gene therapy? A. Lytic vector expression B. Integration into target region C. Immune response to viral vector D. Production of chimera
Which of the following is a known characteristic that can lead to genetic changes in some viruses? Operons in the viral genome can undergo homologous recombination, leading to genetic rearrangements. Reassortment of segmented genomes can occur if a host cell is co-infected with two different viral strains. RNA polymerases used in replication of the viral genome have proofreading capability. Environmental conditions can trigger the SOS response, which triggers mutations in the viral genome.
Ashanti Silva received the first gene therapy for the treatment of SCID. What do you think, would the combination of gene therapy and stem cell therapy be a better therapeutic option?
Some retroviruses that have a wide host range have been developed for use in treatment of diseases or syndromes. These viruses do not result in a productive infection because they lack critical genes for viral replication, but they do carry DNA sequences that allow them to be packaged into infectious particles. Once in the host cell the viral DNA is released and integrates into the cell's genome. The advantage to this strategy is that the delivery of the DNA is very efficient. What is one possible problem of using a virus that can integrate into the human nuclear genome? Multiple Choice The virus carries genes that will interfere with host cell activities. The viral DNA sequences could still cause a disease. Since the viral DNA sequences no longer contain essential genes for viral replication the host cell may not be able to replicate either. The integration could disrupt an essential gene or genes.
Discuss how advances in our understanding of the human genome have improved our understanding of disease Give examples.