Protein vaccines include only the parts (i.e. a protein) of a virus that best stimulate your immune system. This type of COVID-19 vaccine contains harmless S proteins. Once your immune system recognizes the S proteins, it creates antibodies and defensive white blood cells. If you later become infected with the COVID-19 virus, the antibodies will fight the virus. Assume that you work in a project for development of such vaccine against COVID-19. The vaccine contains S Protein and your job includes isolation of pure S Protein from the virus determination of charge on the protein (or pI) determination of molecular mass revealing three dimensional (3-D) structure of the protein How would you isolate (purify) the protein; suggest different techniques and explain the techniques. How would you determine the pI of the protein? Suggest techniques for determination of molecular mass.

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Viral Infections

Viruses are abundantly found, minute entities that have genetic material enclosed within a protein coat. The proteinaceous material enclosing the genetic material of the virus is called a capsid. The genetic material could be either DNA (deoxyribonucleic acid) or RNA (ribonucleic acid). A virus is too minute to be looked at with naked eyes and therefore visualized with the help of a microscope. They are acellular entities that require a host for processes, such as replication, transcription, translation and multiplication. Plants, animals, and bacteria act as host wherein the virus reproduce. Viruses cannot multiply outside the host's body and thus are considered nonliving outside the host body.