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- A student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X objective lenses. The student has a slide with cells that are -100 microns (1 micron = 103 mm) in diameter, and wants to view a magnified image of a %3D cell so that the cell appears to be 10 mm in diameter. Which objective lens should the student use? O 10X O 40X O 100X O 4X Question 7 2 ptsYou have a suspension of cells at a concentration of 5 x 106 cells/mL in culture media. The protocol you’re using for your experiment asks you to dilute the cells 1:10 in fresh culture medium to make a 20 mL suspension. How much of your original suspension and fresh culture media will you need? What’s the concentration of cells now in your working suspension?A student counts 50 cells across the diameter of the field of view, and there are 70 rows of cells. If the diameter of the field of view is 3500 μm, what is the length and width of the cells? Be sure to show all your work.
- Suppose you have a suspension of HeLa cells with a cell count of 85,000 cellsper ml, and you want to prepare a suspension for your experiment that has a cellcount of 40,000 cells per ml. You need 10 mls total for your experiment. Howmany mls of cell suspension will you add to your experimental tube? How manymls of cell culture media to bring the volume up to 10 mls?Volume of cell suspension: _____________________________________Volume of cell culture media:____A student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X objective lenses. The student has a slide with cells that are -100 microns (1 micron = 10 mm) in diameter, and wants to viewa magnified image of a cell so that the cell appears to be 10 mm in diameter. Which objective lens should the student use? 10X O 40X O 100X 0 4XThe actual length of a cell is 50µm. When projected on the big screen in the class, the same cell is 2m long. What is the magnification factor of the cell on the screen? 1 000x 4 000x 10 000x 40 000x 100 000x 400 000x
- Assume that you are starting with a sample containing 6 billion active cells. Create a dilution plan to reduce this count to a range of 30-300 colonies on a plate. The powdered sample weighs 500 mg (0.5 g). You can use up to 4 plates – you should plate different dilutions and/or volumes of solution. Start your first dilution by adding 10 mL diluent to 0.5 g powder, which is a 1/20 dilution. Hint: Rearranging the “cfu/ml” formula from our dilutions lab can help you solve this. Choose a target cfu count, and your volume plated should be under 0.5 ml.Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?You have an original cell density of 4.3x10 8CF/mL. What is this number in its non-scientific notation or "regular" format?. 43,000,000CF/mL. 0.000000043CfU/mL. 430,000,000CFU/mL. 0.00000043CFU/mL. 4.3CFU/mL
- You want to determine the amount of cells in a culture. You dilute the suspension to 10^-4 and plate 100ul onto an agar plate. After overnight incubation there are 30 colonies on your plate. How many cells are in your original suspension (assume your culture is 1 Liter)?Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will use to obtain a cell fraction that consists of mostly large cell organelles: collect pellet, collect supernatant, high-speed centrifugation for 10 min., low-speed centrifugation for 5 min, filter homogenate, grind plant tissue Step 1 grind plant tissue | Choose collect supernatant low-spccd centrifugation for 5 min high-speed centrifugation for 10-min collect pellet grind plant tissue filter homogenate Step Step 3 Step 4 high-speed contrifugation fo vAn ocular reticle measures 1.2 mm in length. At this same magnification, an unknown cell measures 3 r.u. in length. What is the size of this cell in um? Show your calculation and include units.