How do I convert from DPM to picomoles per milligram of protein (pmol/mg)? I have the specific activity as [3H]-spiperone is 25530 DPM/pmol. is there an equation?
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- 16.B. The formula for "Lubow's Solution" is as follows3: 1 g 500 mg Progesterone Hydrocortisone 2 g Propylene glycol Ethanol (95%) ad 100 mL (a) Calculate the quantities of each ingredient required to prepare 2.5 mL of solution. (b) How many milliliters of propylene glycol (sp. gr. 1.04) are needed to prepare a liter of the formula?Which of these heterocyclic drugs is likely to be the least soluble in water? Use the Fsp³ parameter to decide. OH Tramadol Chemical Formula: C16H25NO2 YOUR OW Pantoprazole Torasemide Chemical Formula: C16H15F2N3O4S Chemical Formula: C16H20N4O3S Temazepam -OH Chemical Formula: C16H13CIN₂O2 Tioconazole Chemical Formula: C16H13C3N₂OS A. Tramadol B. Pantoprazole C. Torasemide D. Temazepam E. Toconazole27. The standard-state free energy of hydrolysis for acetyl phosphate in 1,3-Bisphosphoglycerate is 1,3-Bisphosphoglycerate + H₂O 3-phosphoglycerate + Pi AGI-49.6 kJ/mol. Determine the free energy change for 1,3-Bisphosphoglycerate hydrolysis in a solution of 5.4 mM 3-phosphoglycerate, 0.0054 M inorganic phosphate, and 20 nM 1,3-Bisphosphoglycerate.
- 80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pI. The solution is then titrated with 0.2M HCL to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCL needed to reach relevant pKa value and equivalence point(s)z Relevant pKa values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.The value of AG®' for the conversion of 3-phosphoglycerate to 2-phosphoglycerate (2PG) is +4.40 kJ/mol. If the concentration of 3-phosphoglycerate at equilibrium is 1.95 mM, what is the concentration of 2-phosphoglycerate? Assume a temperature of 25.0 °C. [2PG] = mM80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pl. The solution is then titrated with 0.2M HCI to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCl needed to reach each relevant pKa value and equivalence point(s). Relevant pka values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.
- Acetocholinesterase is an enzyme possessing a single active site that metabolizesacetylcholine with a turn over number of 1.4 x 10^4s-1. How many grams of acetylcholine(molecular formula C7NO2H16+) will 2.16 x 10^-6 g acetocholinesterase metabolize in 60minutes? (The enzyme’s molecular mass is 4.2 x 10^4 g/mol).The standard state free energy of hydrolysis of acetyl phosphate isΔG° = -42.3 kJ/mol. Acetyl-P + H2O acetate + Pi Calculate the free energy change for the acetyl phosphate hydrolysis in a solution of 2 mM acetate, 2 mM phosphate and 3 nM acetyl phosphate.5) In an experiment to investigate the inhibition of the enzyme-glucosidase the following data for the rates of reaction with glucopyranoside for various substrate concentrations was obtained. By constructing a Leaver-Burk plot, determine the value of the Michaelis constant. [S]/ (10-6 mol dm-3) v/ (10-3 mol dm-3 s-1) 1.00 2.00 3.00 4.00 16.7 33.3 41.1 49.8
- Assume that the reaction Arabinose +Pi <> Arabinose-6-P has a delta G of +13.0 kJ/mol and the reaction XTP <> XDP + Pi has a delta G of -25.7 kJ/mol. Calculate the delta G for the combined reaction Arabinose + XTP <> Arabinose-6-P + ADP, in kJ/mol to four significant figures.The equilibrium constant Kc for the reaction fructose-1,6-diphosphate ⇋ glyceraldehyde-3-phosphate + dihydroxyacetone phosphate is 8.9 x 10-5 M at 250C and the behavior is assumed to be ideal. Calculate the delta G for the process Suppose that we have a mixture that is initially 0.010 M in fructose-1,6-diphosphate and 1.0 x 10-5 M in both glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. What is the delta GQuinine ( C20 H24 N2 O2) is the most important alkaloid derived from cinchona bark. It is used as an antimalarial drug. For quinine, pK, = 5.1 and pK, = 9.7 ( pKp = – log Kp). Only 1 g quinine will dissolve in 1920.0 mL of solution. Calculate the pH of a saturated aqueous solution of quinine. Consider only the reaction | Q+ H2O= QH+ + OH- described by pK, where Q = quinine. pH =