Experiment 2: Determining the effect of pH on Fungal Amylase Activity To investigate the effect of pH on amylase activity, we mixed 6 ml of 0.5% amylose solution buffered at a specific pH level with 70ul of 0.5% amylase enzyme solution. We investigated pH levels of 3, 4, 5, 6, 7, and 8. At 15 second intervals, we tested the reaction mixture for the presence of amylose starch by dropping the mixture into a well containing a drop of Lugol's iodine. We compared our results against the following: a well that contained only amylase solution and iodine, a well containing only enzyme solution and iodine, and a well containing completely digested (or broken-down) starch solution and iodine. We recorded the time of completion of the reaction for each of our trials, when no more starch was present, as the first sample that did not show a positive starch reaction. If the reaction did not reach completion in 9 minutes, a time of >540 seconds was recorded, and notes were taken about whether or not some breakdown of starch appeared to have happened. 13. Should pH affect the time it takes the reaction to reach completion? Why?

Experiment 2: Determining the effect of pH on Fungal Amylase Activity To investigate the effect of pH on amylase activity, we mixed 6 ml of 0.5% amylose solution buffered at a specific pH level with 70ul of 0.5% amylase enzyme solution. We investigated pH levels of 3, 4, 5, 6, 7, and 8. At 15 second intervals, we tested the reaction mixture for the presence of amylose starch by dropping the mixture into a well containing a drop of Lugol's iodine. We compared our results against the following: a well that contained only amylase solution and iodine, a well containing only enzyme solution and iodine, and a well containing completely digested (or broken-down) starch solution and iodine. We recorded the time of completion of the reaction for each of our trials, when no more starch was present, as the first sample that did not show a positive starch reaction. If the reaction did not reach completion in 9 minutes, a time of >540 seconds was recorded, and notes were taken about whether or not some breakdown of starch appeared to have happened. 13. Should pH affect the time it takes the reaction to reach completion? Why?

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter13: Dimensional Analysis/units Conversion

Section: Chapter Questions

Problem 1.3P

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