Dr Merrick is so excited to know the affinity constant Kd values for O2 binding to mammoth OBP and elephant hemoglobin at pH=7, that he starts speculating about the kinetics of binding to O2. If the ka value of mammoth EHb-O2 complex was the same as the ka value of mammoth OBP-O2 complex, what would be the ratio between the kd values of these complexes? Explain your reasoning. Hint: we are looking for the ratio kd(EHb)/kd(OBP) at pH=7.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
) Dr Merrick is so excited to know the affinity constant Kd values for O2 binding to mammoth OBP and elephant hemoglobin at pH=7, that he starts speculating about the kinetics of binding to O2. If the ka value of mammoth EHb-O2 complex was the same as the ka value of mammoth OBP-O2 complex, what would be the ratio between the kd values of these complexes? Explain your reasoning.
Hint: we are looking for the ratio kd(EHb)/kd(OBP) at pH=7.
![Question 1) Dr John Merrick is characterizing a protein from a gene that he cloned from a frozen mammoth
tissue. Since this protein has the ability to bind oxygen, Dr Merrick called the protein OBP (oxygen binding
protein). To characterize OBP, he decided to compare its 02 binding ability with that of elephant hemoglobin.
He did the experiment at normal pH=7 and at lower pH=6.5.
Elephant Hb (pH=7)
Mammoth OBР (рн-7)
100
Elephant Hb
(PH36.5)
75
Mammoth OBP
50
(pH=6.5)
25
10
20
30
40
02 [µM]*
saturation (%)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fb38f11b4-2041-4b9a-99fb-b8e390e6e98c%2F7c10e4a9-e80b-47ce-90bf-93d89678af92%2Fwhq1stg_processed.png&w=3840&q=75)
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