Below is a list of "solutions" to problems encountered when bacteria replicate DNA. Match the "solution" to the specific "problem" encountered during DNA replication ligase [ Choose ] [Choose ] helicase conversion of ds DNA into ss templates relief of chromosome supercoiling Primase synthesis of short primer replacement of RNA with DNA

Below is a list of "solutions" to problems encountered when bacteria replicate DNA. Match the "solution" to the specific "problem" encountered during DNA replication ligase [ Choose ] [Choose ] helicase conversion of ds DNA into ss templates relief of chromosome supercoiling Primase synthesis of short primer replacement of RNA with DNA

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter11: Genome Alterations: Mutation And Epigenetics

Section: Chapter Questions

Problem 18QP: Replication involves a period of time during which DNA is particularly susceptible to the...

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During plasmid extraction, after the addition of acetate-conta in ing neutralization buffer the large DNA and prote ins precipitate, but the small bacterial DNA plasmids can renature and stay in solution. Autosome O Plasmid O Gen omic OMitotic O Karyotype
In a typical microbiology laboratory, reasons for no bands from a gel of a polymerase chain reaction may bedue to errors relating to omission of ingredients in the reaction mix and absence of the target sequence inthe template DNA. Based on (i) primer problem and (ii) purity/potential contamination of the target sequence, explain the reason for non-appereance on bands.
You are cloning the genome of a new DNA virus into pUC18.You plate out your transformants on ampicillin plates containing X-gal and pick one blue colony and one white colony.When you check the size of the inserts in each plasmid (blueand white), you are surprised to fi nd that the plasmid fromthe blue colony contains a very small insert of approximately60 bp, while the plasmid from the white colony does notappear to contain any insert at all. Explain these results.
Which of the following mechanisms does not contribute to reducing the overall in vivo mutation rate found in most species? Mismatch repair of incorrectly inserted bases Replication-associated proteins, in addition to DNA polymerase, aid in fidelity of replication O Excision repair of thymine dimers O Tautomerization of nitrogenous bases Proofreading by DNA polymerase's 3' to 5' exonuclease activity
In a standard procedire, when writing and reading base sequences for nucleic acids (both DNA and RNAs) always to specify base sequence in 5' > 3' direction unless otherwise directed 1. From the base sequence 5' A-T-G-C-C-A 3' in a DNA template strand, determine the base sequence in hnRNA synthesized from the DNA template strand 2. From the base sequence 5' T-A-A- C-C-T 3' in a DNA template strand, determine the base sequence in hnRNA synthesized from the DNA template strand
In bacterial cells, nucleotide excision repair involves which of the following proteins? DNA glycosylase AP endonuclease photolyase AlkB UvrABC proteins If Meselson and Stahl had results from density gradient analysis of bacterial DNA that indicated only two bands, one of the original density and one that was the same as unlabeled DNA, and no intermediate density band, this would indicate that DNA replication is: constructive semiconservative conservative consecutive cannot determine from the information given 6.In E. coli, which DNA polymerase is primarily responsible for filling in the gaps in the DNA generated during nucleotide excision repair? DNA polymerase I DNA polymerase II DNA polymerase IV DNA polymerase V none of the above
Match the following (most appropriate combinations): helicase Topoisomerase DNA Polymerase III DNA Polymerase I Primase [Choose ] Replicates bacterial chromosomes Separates DNA strands Changes Supercoiling Has a 5-to-3 exonuclease activity RNA synthesis Changes Supercoiling Has a 5-to-3 exonuclease ac RNA synthesis
vvnicn the following statements are correct about the repair of a DNA duplex containing the sequence below that is grown INE coli (select all that apply)? Strand A Strand B GATCTAGCCGGCATCCGAT CTAGATCGGACGTAGGCTA Methyl ✔A. MutH cleaves Strand A O B. DNA repair will result in the bold A in strand B being replaced with a C O C. DNA repair will result in the bold G in strand A being replaced with a T ✔ D. Defect will not be properly repaired in dam(-) E coli O E. The mammalian repair system would also correct the mismatch shown based on the methylation status of the DNA
In the very first round of PCR using genomic DNA,the DNA primers prime synthesis that terminates onlywhen the cycle ends (or when a random end of DNA isencountered). Yet, by the end of 20 to 30 cycles—a typicalamplification—the only visible product is defined pre-cisely by the ends of the DNA primers. In what cycle is adouble-stranded fragment of the correct size first gener-ated?
BamHI site BamHI site kanT 3755,6p 784 bp PAMP 4539 bp 2332 pp PKAN 4207 bp HindllI site 1875 bp amp HindlII site Origins of replication You are using these two plasmids to make a recombinant DNA molecule. You want to make a molecule, that has two origins of replication and is amp but NOT kanR, using only one fragment from each plasmid. How large, in base pairs, will be your desired recombinant DNA molecule? O 6087 base pairs O 5630 base pairs O 2659 base pairs O 8746 base pairs 47 8 24 & 4. 8. 9. 4 5 6 RI T 13 H. 2 K FL
22.124 Give two reasons why bacterial cells am wred for recombinant DNA procedures. Nucleic Acids 1015 22.125 What role do plasmids play in recombinant Polymerase Chain Reaction (Section 22.15) 22.131 What is the function of the polymerase chain DNA procedures? 22.126 Describe what occurs when a particular restric- reaction? tion enzyme operates on a segment of double- stranded DNA. 22.127 Describe what happens during transformation. 22.128 How are plasmids obtained from E, coli bacte- 22.132 What is the function of the enzyme DNA polymerase in the PCR process? 22.133 What is a primer and what is its function in the PCR process? 22.134 What are the four types of substances needed to carry out the PCR process? ria? 22.129 A particular restriction enzyme will cleave DNA Sequencing (Section 22.16) DNA between A and A in the sequence AAGCTT in the 5'-to-3' direction. Draw a dia- gram showing the structural details of the "sticky ends" that result from cleavage of the following DNA segment.…
Escherichia coli's chromosome has a replication origin called OriC. Draw a schematic diagram to show the specific DNA sequences that is essential for replication function scattering over a 245 base pair of Oric region.