Bacterial growth depends on many environmental factors, including the temperature of the environment. Since microbes can survive in a certain range of temperatures and will thrive at a temperature, understanding these variables allows for control of their growth. This understanding can be used, for example, to preserve certain foods or to treat infections. This lab simulation will use an instrument called a spectrophotometer. This instrument quantitatively measures the amount of light that is absorbed or transmitted by molecules in solution. Microbial populations grow at different rates based on temperature. One way to measure growth is by turbidity measurements. When bacteria multiply in a broth culture they turn the clear solution cloudy, or turbid. a) in your own words explain the proper steps to Inoculate 5°C sterile broth tube using aseptic technique

Bacterial growth depends on many environmental factors, including the temperature of the environment. Since microbes can survive in a certain range of temperatures and will thrive at a temperature, understanding these variables allows for control of their growth. This understanding can be used, for example, to preserve certain foods or to treat infections. This lab simulation will use an instrument called a spectrophotometer. This instrument quantitatively measures the amount of light that is absorbed or transmitted by molecules in solution. Microbial populations grow at different rates based on temperature. One way to measure growth is by turbidity measurements. When bacteria multiply in a broth culture they turn the clear solution cloudy, or turbid. a) in your own words explain the proper steps to Inoculate 5°C sterile broth tube using aseptic technique

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter42: Basic Microbiology

Section: Chapter Questions

Problem 2CR

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Bacterial growth depends on many environmental factors, including the temperature of the environment. Since microbes can survive in a certain range of temperatures and will thrive at a temperature, understanding these variables allows for control of their growth. This understanding can be used, for example, to preserve certain foods or to treat infections. This lab simulation will use an instrument called a spectrophotometer. This instrument quantitatively measures the amount of light that is absorbed or transmitted by molecules in solution a) In your own words give an introduction to the microbial growth and the effect tempature has. b) In your own words explain the importance of the spectrophotometer in regards to microbial growth. c) In your own words give a hypothesis on how bacteria will react to different temperatures.
As part of an experiment where absorbance values are measured using a spectrophotometer, you are taking readings of your sample every 20 minutes. The non-motile microbe you're testing has a generation time of roughly 20 minutes at an incubation temperature right around room temperature. Things start out fine, with the expected results — as time goes by at the correct incubation temperature, absorbance starts to rise as the medium starts to become more cloudy with growing microorganisms. But roughly 2 hours into the process, you notice that the absorbance levels flatten out, and then start to decrease unexpectedly. What is most likely taking place in your experiment?
This diagram shows the filter paper method used to evaluate the inhibitory effect of chemical agents, heavy metals, and antibiotics on bacterial growth. A culture of a test bacterium is spread uniformly over the surface of an agar plate. Small filter paper discs containing the material to be tested are then placed on the surface of the medium. A disc that has been soaked in sterile distilled water is sometimes added as a control. After incubation, a lawn (film of growth) will cover the plate, but a clear zone will surround those discs that contain an inhibitory compound. The size of the zone reflects several factors, one of which is the effectiveness of the inhibitory agent. What are two other factors that might affect the size of the zone of inhibition? What is the purpose of the control disc? If a clear area were apparent around the control disc, how would you interpret the observation?
There are various environmental factors that can influence microbial growth and reactions. These factors play a role which will determine if a microbe can function in a given environment. What are 2 environmental factors believed to be the most important out of the others ?
A lab technician is working with a bacterium in pure culture (in 5 ml of liquid media in a test tube). The bacterium is a mesophile that can infect humans. Which of the following is NOT true (with regards to temperature conditions for this bacterial culture)? Lowering the temperature to -10 deg C for at least an hour will likely kill all the bacteria. Placing the tube at 37 deg C will likely facilitate rapid growth of the bacteria. Raising the temperature to 90 deg C for at least an hour will likely kill all the bacteria. Placing the tube at 4 deg C will likely slow or halt growth of the bacteria.
After quick-characterization by staining, specialized growth media can be used to indicate further biochemical characteristics of an unknown organism. These tests use visual outcomes, such as colors, patterns, and changes in appearance. The results can be interpreted to then assign a characteristic to the unknown organism. For example, a color change may happen when an unknown organism is incubated in a mixed sugar broth. The color could indicate which sugar was (or was not) used by the bacterium for energy. a) explain in your own words how to perform specialized media tests to find an unknown bacteria
You perform a ten-fold serial dilution of a bacterial culture to determine the number of colony forming units (CFU) per mL in the culture. You then do a plate count with these growth results (no. of colonies): 1:10: too many to count; 1:100 too many to count; 1:1,000: 126 colonies; 1:10,000 14 colonies; 1:100,000: no growth The number of CFU per mL in the original culture was: 1 ml 1 ml 1 ml 1 ml Original inoculum Dilutions 1,000 126 9 ml broth in each tube 126,000 10,000 140,000 1:10 1 ml 1:100 1 ml 1:1000 1 ml 1 ml 1:10,000 1 ml 1:100,000 1 ml
In microbiology, the term growth in a culture usually refers to an increase in O 1) the number and size of microbial cells. O 2) the amount of ATP consumed. O 3) a microbe's size. O 4) the number of microbial cells. 5) the amount of ATP produced.
Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is one experiment you could do, involving culturing the organism?
propose a hypothesis regarding the organisms (staphylococcus, escherichia, pseudomonas, and bacillus) that will be resistant to the most disinfectants, and why? if the hypothesis is supported, what specific experimental results will be observed? what would be the independent and dependent variable? zone size, disinfectant type, colony count
On which of the following types of media would you expect untransformed bacteria to grow on? LB agar LB agar with ampicillin Untransformed bacteria will not grow on any type of medium. Untransformed bacteria will grow on all three types of media. LB agar with ampicillin and arabinose
Which of the following statements regarding microbial control agents is FALSE? Question options: strongly acidic or alkaline disinfectants are typically more effective than neutral pH agents the presence of extracellular fats typically decreases the effectiveness of control agents a warmed disinfectant is typically more effective than the same disinfectant at room temperature substances secreted by members of biofilms increase protection against microbial control agents all of these statements are TRUE bacterial endospores are more resistant to antimicrobial treatment than vegetative bacterial cells