a. The scale of a spectrophotometer extends from 1 to 100% T, what are the values of these two extremes in absorbance? b. Convert the 98%T to absorbance
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a. The scale of a spectrophotometer extends from 1 to 100% T, what are the values of these two extremes in absorbance?
b. Convert the 98%T to absorbance
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- Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's LawConsider a bi convex lens with radius of curvatures |R1| = 30 mm and |R2| = 180 mm, ct = 5 mm and glass is N-BK7. This is used as camera lens in order to image a man who is at 1 km away from the camera. We want to resolve 10 cm on his body. Assume that λ = 550 nm. Using zemax, determine the MTF value for the resolution of R = 80 lp / mm in image plane which is placed at paraxial focus. (Hint: First, find the diameter of the lens) Which one: a.32.1 % b.28.7 % c.12.3 % d.58.5 % e.46.8 % f.57.2 % g.20.9 % h.15.6 %the figure shows the result of the spectral analyzer for paper spectrophotometer without any transparent paper and with transparent yellow color paper. observe and discuss the intensity and the wavelength for both results.
- Given the following absorbance spectrum, to what wavelength should you set the spectrophotometer to measure your samples? Absorbance 400 500 600 Wavelength (nm) 700if the absorbance value measured in a spectrophotometer is 0.5, how much of the original incident light is being measured by the detector?What is the reason for the decrease in the ABS value over time when measuring in the spectrophotometer?
- If a researcher forgot to wipe the outside a sample cuvette to make it free of drops, fingerprints, and dust prior to reading it in the spectrophotometer, how might the absorbance value of the sample be affected? (Higher, Lower, No Change) ExplainThe field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?What is the diameter of the field of view (ie. Diameter2) when the total magnification is 100X? Report your answer in µm
- In spectrophotometry, what is the role of the green background in the set-up? What does it represent in the actual spectrophotometer?The FOV on low power of a microscope is measured to be 4.5mm. Using the chart below and the above listed formulas calculate: FOvx = FOvlow X Maglow Magx S.S. = FOvx # times the specimen fits across the FOv Total Mag = Ocular lens x objective lens a) What is the FOV on High Power? (Magnification on High power is 40x) Can you please include the units too. asap please.Activity: Calculate the magnification for the following. Actual Specimen Size Image Size Magnification 0.5 μm 2 cm 200 μη 1 cm 10 cm 25 x 4 cm 50 x 4 mm 3 x