6. Today we amplified 150 ng of Bos taurus (calf) DNA by PCR. This amount of DNA contains about 46324 molecules of the insulin gene [150 ng DNA= 7.69 x 10-20 mol; (7.69x10-20 )x(6.023×1023) = 46324 molecules]. performed PCR for 35 cycles to amplify the amount of this gene. a. What is the theoretical fold amount of DNA amplified by 35 cycles of PCR (remember the 2N formula)?
6. Today we amplified 150 ng of Bos taurus (calf) DNA by PCR. This amount of DNA contains about 46324 molecules of the insulin gene [150 ng DNA= 7.69 x 10-20 mol; (7.69x10-20 )x(6.023×1023) = 46324 molecules]. performed PCR for 35 cycles to amplify the amount of this gene. a. What is the theoretical fold amount of DNA amplified by 35 cycles of PCR (remember the 2N formula)?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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![Today we amplified 150 ng of Bos taurus (calf) DNA by PCR. This amount of DNA contains about 46324
molecules of the insulin gene [150 ng DNA= 7.69 x 10-2⁰ mol; (7.69x10-20 )x(6.023×10²³) = 46324 molecules].
performed PCR for 35 cycles to amplify the amount of this gene.
a. What is the theoretical fold amount of DNA amplified by 35 cycles of PCR (remember the 2N formula)?
6.
b. How many molecules of the insulin gene would therefore be present after PCR?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fcf8c917b-7d25-4fea-9b3f-90a987505563%2Fce52aa9b-16d7-4a07-b91a-f6b966497013%2F9fn1xit_processed.png&w=3840&q=75)
Transcribed Image Text:Today we amplified 150 ng of Bos taurus (calf) DNA by PCR. This amount of DNA contains about 46324
molecules of the insulin gene [150 ng DNA= 7.69 x 10-2⁰ mol; (7.69x10-20 )x(6.023×10²³) = 46324 molecules].
performed PCR for 35 cycles to amplify the amount of this gene.
a. What is the theoretical fold amount of DNA amplified by 35 cycles of PCR (remember the 2N formula)?
6.
b. How many molecules of the insulin gene would therefore be present after PCR?
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