Chapter 28, Problem 1AQ

Define the procedures you would use to isolate and identify a new pathogen. Keep in mind Koch’s postulates (Figure 1.29) as you form your answer. Be sure to include growth-dependent assays, immunoassays, and molecular assays. Which of your assays could be adapted to be used as a routine, high-throughput test for rapid clinical diagnosis?

Summary Introduction

To explain:

The procedure used to isolate and identify a new pathogen and the method would be more suitable to the high throughput test for rapid clinical diagnosis with perspective to growth dependent assays, immunoassays and molecular assays

Introduction:

Robert Koch a German microbiologist and physician is the father of modern microbiology. He made several achievements which include identification of key agents in tuberculosis, anthrax and cholera disease types. His postulates led a way to determine and identification of microorganisms based on morphology and key characteristics. He was awarded the Nobel Prize in 1905.

Explanation of Solution

A procedure used to isolate and identify the new pathogen is done by direct observation and culture method. Moreover, the pathogen is observed through direct observation of sputum and urethral exudates in infectious diseases. They include acid-fast stained Mycobacterium tuberculosis and Neisseria gonorrhea. Most of the pathogens are grown in cultures in the laboratory. In contrast, clinically important bacteria are grown and isolated in growth media.

Clinical diagnosis of growth dependent assays is used to report the findings. Depending upon the growth on enriched media, the pathogen is sub-cultured on media, which is designed to measure biochemical reactions. The media used can be differential or selective.