Foundations in Microbiology
9th Edition
ISBN: 9780073522609
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 10.L1, Problem 1MCQ
Which gene is incorporated into plasmids to detect recombinant cells?
a. restriction endonuclease
b. virus receptors
c. a gene for antibiotic resistance
d. reverse transcriptase
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Which gene is incorporated into plasmids to detect recombinantcells?a. restriction endonuclease
b. virus receptors
c. a gene for antibiotic resistance
d. reverse transcriptase
involved in the transfer of multiple drug resistance from one cell to another? *
a.Transposition
b. Conjugation with a cell with chromosomal drug resistance appears in the genome of a bacteriophage that has infected it.
c. Transformation of chromosomal genes
d. Conjugation with a cell with a free plasmid carrying drug resistance
What is used to cut the DNA in panels 1 and 2?
a.
Restriction enzymes
b.
DNA polymerases
c.
DNA ligases
d.
RNA polymerases
e.
Reverse transcriptase
Chapter 10 Solutions
Foundations in Microbiology
Ch. 10.1 - Define genetic engineering, and describe some of...Ch. 10.1 - Explain the properties of DNA that lend to its...Ch. 10.1 - Summarize the major methods of analyzing DMA and...Ch. 10.1 - Describe the technology behind Identifying,...Ch. 10.1 - Define genetic engineering and biotechnology, and...Ch. 10.1 - Describe the processes involved in denaturing and...Ch. 10.1 - Define restriction endonuclease and explain what...Ch. 10.1 - Prob. 4CYPCh. 10.1 - Prob. 5CYPCh. 10.1 - How would you make a copy of DNA from an mRNA...
Ch. 10.1 - Briefly summarize the steps involved in DNA...Ch. 10.1 - Outline the steps in the PCR technique and...Ch. 10.1 - What are the functions of primer and Taq...Ch. 10.2 - Explain what is involved in recombinant DNA...Ch. 10.2 - Characterize the events in cloning, using an...Ch. 10.2 - List and discuss some protein products of...Ch. 10.2 - What characteristics of plasmids and...Ch. 10.2 - Name several types of vectors, and list the types...Ch. 10.2 - Describe the basic principles behind recombinant...Ch. 10.2 - Summarize the characteristics of bacteria and...Ch. 10.2 - Outline the main steps in cloning a gene,...Ch. 10.2 - What is one way to determine whether a bacterial...Ch. 10.2 - Characterize several products that have resulted...Ch. 10.3 - Define what is meant by the term transgenic or...Ch. 10.3 - Describe the uses of genetically modified bacteria...Ch. 10.3 - Prob. 10ELOCh. 10.3 - Explain how DNA technology can be used to treat...Ch. 10.3 - Describe several uses of genetically modified...Ch. 10.3 - Prob. 18CYPCh. 10.3 - Why must animals usually be modified in the embryo...Ch. 10.3 - Prob. 20CYPCh. 10.3 - What are some ethical and biological...Ch. 10.4 - Outline the uses of gene therapy and gene editing...Ch. 10.4 - Prob. 14ELOCh. 10.4 - Describe several applications of DNA profiling and...Ch. 10.4 - Describe what a DNA fingerprint is and why and how...Ch. 10.4 - Prob. 24CYPCh. 10.4 - Explain the origins of mtDNA and its importance in...Ch. 10.4 - Explain the difference between a DNA profile and a...Ch. 10.L1 - Which gene is incorporated into plasmids to detect...Ch. 10.L1 - Which of the following is not essential to carry...Ch. 10.L1 - Which of the following is not a part of the Sanger...Ch. 10.L1 - The function of ligase is to a. rejoin segments of...Ch. 10.L1 - The pathogen of plant roots that is used as a...Ch. 10.L1 - Prob. 6MCQCh. 10.L1 - Which DNA fragment will be closest to the top...Ch. 10.L1 - Prob. 8MCQCh. 10.L1 - For which of the following would not require a...Ch. 10.L1 - Prob. 10MCQCh. 10.L1 - What type of mutation caused Nicholas’s disease?...Ch. 10.L1 - Which type of cells were used to extract the DNA...Ch. 10.L1 - Lay out the genetics of Nicholas’s case,...Ch. 10.L1 - a. Why do bacteria make restriction endonucleases?...Ch. 10.L1 - a. Using nucleotide letters, show the kind of cut...Ch. 10.L1 - Prob. 3WCCh. 10.L1 - a. Explain what hybridization is and how it is...Ch. 10.L1 - Prob. 5WCCh. 10.L1 - Prob. 6WCCh. 10.L1 - Prob. 7WCCh. 10.L1 - Explain the kinds of study involved in genomics,...Ch. 10.L2 - a. Give an example of a benefit of genetic...Ch. 10.L2 - a. When gene probes, fingerprinting, and...Ch. 10.L2 - Which suspect is the likely perpetrator according...Ch. 10.L2 - a. Describe how a virus might be genetically...Ch. 10.L2 - You are on a jury to decide whether a person...Ch. 10.L2 - a. Explain how the fish and game department, using...Ch. 10.L2 - What are some of the major impediments to...Ch. 10.L2 - Prob. 8CTCh. 10.L2 - Describe the main differences between genome...Ch. 10.L2 - Below are two unrelated DNA paternity tests: one...Ch. 10.L2 - Figure 9.25d, shown here, shows the original...
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- Which type of cells were used to extract the DNA that was sequenced? a. red blood cells c. white blood cells b. intestinal epithelium d. cheek swab What type of mutation caused Nicholas’s disease? a. frameshift c. nonsense b. missense d. insertionarrow_forwardafter a successful infection by a viral particle, it integrates its genome into the host DNA. which term best describe the change of host genome? a. none b.silent mutation c.missense mutation d.frame shift mutationarrow_forwardWhich type of gene therapy involves the direct administration of viruses in patients' tissues? a. in vitro gene therapy b. in vivo gene therapy c. ex vitro gene therapy d. ex vivo gene therapyarrow_forward
- What is the role of a virus in gene therapy?a. It causes the disease that gene therapy is aiming to cure.b. It carries the healthy DNA into the patient’s cells.c. It carries the faulty DNA out of the patient’s cells.d. It reveals which cells carry the DNA causing the disease.arrow_forwardWhat technique can be used for studying the gene for cystic fibrosis? a. cellular cloning b. molecular cloning c. reproductive cloningarrow_forwardWhich nucleic-acid based technique will detect norovirus? A. Fluorescent In Situ Hybridization (FISH) probes B. PCR with primers specific for the genome of this virus C. Terminal Restriction Fragment Length Polymorphism (T-RFLP) D. reverse transcription PCR with specific primers A patient's blood sample tests positive for the antigen to Vibrio cholerae. Which of the following interpretations is correct? A. This person has cholera. B. This person was exposed to any species of Vibrio. C. This person was exposed to V. cholerae recently. D. This person had cholera at some point in their lifetime.arrow_forward
- In which bacteriophage life cycle does the phage DNA become incorporated into the bacterial chromosome? a. Lytic b. Lysogenic c. Both lytic and lysogenic d. Neither lytic nor lysogenicarrow_forwardMatch the process to the step in producing a transgenic plant using the Ti plsmid method. 45. A gene is isolated and the process starts. 46. The recombinant DNA has this so it can be found later. 47. The plasmid enters here. 48. A. tumefaciens infects target organism. 49. The selectable marker allows transgenic plants to survive antibiotic. 50. Transgenic plant cells are isolated. 51. Transgenic plants are prepared for life in the soil. Kanamycin is added to culture. b. The isolated transgenic cells are grown into multicellular organisms. C. Ti plasmid picks up gene of interest. d. Bacterium Agrobacterium tumefaciens takes this up. A selectable marker makes the cell resistant to kanamycin. a. e. f. Antibiotics kill any remaining bacteria. g. The host plant is selected and exposed to the new DNA.arrow_forwardWhich Class I element has likely experienced the greatest accumulation of mutations compared to its retrovirus ancestor, as its DNA sequence is the most divergent? A. LINES B. transposons C. Ac/Ds elements D. SINES E. LTR retrotransposonsarrow_forward
- What is a biomarker? a. the color coding of different genes b. a protein that is uniquely produced in a diseased state c. a molecule in the genome or proteome d. a marker that is genetically inheritedarrow_forwardWhat is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forwardExplain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.arrow_forward
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