You design primers that will amplify a 400 bp fragment of the CAT gene and perform PCR. The 400 bp CAT fragment is shown below. The locations where the designed primers anneal are bolded and underlined. 5'GGATTTGGTCGTATTAGGCCTTCTTTTGCGTCGCCAGCCGAGCCACATCGCTCAGACACCATGGGGAAGTG AAGGTCGGAGTCAACGGATTTGGTCGTTTCTTTTGCGTCGCCAGCCGAGCCACATCGCTCAGACACATGGGGA AGTGAAGGTCGGAGTCAACACACTGGGTCATCCCGTCGCGCCTGGTCACCAGGGCTGCTTTTAACTCTGTTCG ACAGTCAGCCGCATCGGAAAGTGGATATTGTTGCCATCAATGACATTGGGCGCCTGGTCACCAGGGCTGCTTT ТААСТСТGGAAAGTGGATAТTGTTGCCATСААTGACCCOТТСАТТGACСТСААСТАСАTGGTTTACATGTTC AATAGATTCCACCCATGAGCCGAGCCACATCGCTCAG -3' a. Write the forward primer sequence, indicate 5' and 3' ends b. Write the reverse primer sequence, indicate 5' and 3' ends

You design primers that will amplify a 400 bp fragment of the CAT gene and perform PCR. The 400 bp CAT fragment is shown below. The locations where the designed primers anneal are bolded and underlined. 5'GGATTTGGTCGTATTAGGCCTTCTTTTGCGTCGCCAGCCGAGCCACATCGCTCAGACACCATGGGGAAGTG AAGGTCGGAGTCAACGGATTTGGTCGTTTCTTTTGCGTCGCCAGCCGAGCCACATCGCTCAGACACATGGGGA AGTGAAGGTCGGAGTCAACACACTGGGTCATCCCGTCGCGCCTGGTCACCAGGGCTGCTTTTAACTCTGTTCG ACAGTCAGCCGCATCGGAAAGTGGATATTGTTGCCATCAATGACATTGGGCGCCTGGTCACCAGGGCTGCTTT ТААСТСТGGAAAGTGGATAТTGTTGCCATСААTGACCCOТТСАТТGACСТСААСТАСАTGGTTTACATGTTC AATAGATTCCACCCATGAGCCGAGCCACATCGCTCAG -3' a. Write the forward primer sequence, indicate 5' and 3' ends b. Write the reverse primer sequence, indicate 5' and 3' ends

Name: You design primers that will amplify a 400 bp fragment of the CAT gen
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Description: You design primers that will amplify a 400 bp fragment of the CAT gene and performPCR.The 400 bp CAT fragment is shown below. The locations where the designed primers anneal arebolded and underlined.5'GGATTTGGTCGTATTAGGCCTTCTTTTGCGTCGCCAGCCGAGCCACAT

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 1ITD: Below is a sequence of 540 bases from a genome. What information would you use to find the...

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