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Solved in 2 steps
- Describe the difference between batch and continuous culture techniquesWrite a note on different culturing, metabolic and physiological techniques used in the Microbiology Lab for culturing as well as identification of microorganisms.Please make an aseptic technique and pure culture technique flowchart
- Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture can be stored.Enumerate the step-by-step procedures of performing both techniques using a schematic diagram. - Pour Plate Technique - Spread Plate TechniqueIf a spectrophotometer is available, dispense the culture into labeled corvette and determine the optical density of each culture
- Distinguish batch culture and continuous cultureIndicate the correct way of inoculating the following media. Plate Media For sensitivity testing ________________________________________________ For isolation of colonies ___________________________________________________In the conduct of serial dilution, which of the following tools is NOT necessary? a. Agar plates b. Micropipettor c. Inoculating needle d. Alcohol lamp or Bunsen burner e. Test tubes or microcentrifuge tubes
- Which of the following is the proper technique for inoculating an agar slant with a broth culture? A. Stab the butt of the media with the wire loop. B. Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop. C. Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant. D. Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.A culture medium that does not grow gram-positive organisms would be a differential plate enrichment plate selective plate streak plate pour platedescribe the agar-overlay technique