Write a step-by-step procedure for this serial dilution. • The stock concentration of the blue dye standard is 1.00 mg/mL. • Serial dilution series from the stock standard with the following final concentrations: 500 ug/mL, 250 ug/mL, 125 ug/mL, 62.5 ug/mL. and a blank that is Oug/ml. • Each standard should have a volume of 1 mL in H2O.

Write a step-by-step procedure for this serial dilution. • The stock concentration of the blue dye standard is 1.00 mg/mL. • Serial dilution series from the stock standard with the following final concentrations: 500 ug/mL, 250 ug/mL, 125 ug/mL, 62.5 ug/mL. and a blank that is Oug/ml. • Each standard should have a volume of 1 mL in H2O.

Chemistry: The Molecular Science

5th Edition

ISBN:9781285199047

Author:John W. Moore, Conrad L. Stanitski

Publisher:John W. Moore, Conrad L. Stanitski

Chapter3: Chemical Reactions

Section: Chapter Questions

Problem 87QRT: You need 300. mL of 0.500-M K2Cr2O7. Which method is best to prepare this solution? Explain your...

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Patients undergoing an upper gastrointestinal tract laboratory test are typically given an X-ray contrast agent that aids with the radiologic imaging of the anatomy. One such contrast agent is sodium diatrizoate, a nonvolatile water-soluble compound. A 0.378-m solution is prepared by dissolving 38.4 g sodium diatrizoate (NaDTZ) in.l.60 102 mL water at 3 1.2C (the density of water at 31.2C is 0.995 g/cm3). What is the molar mass of sodium diatrizoate? What is the vapor pressure of this solution if the vapor pressure of pure water at 31.2C is 34.1 torr?
You need 300. mL of 0.500-M K2Cr2O7. Which method is best to prepare this solution? Explain your choice. (a) Dilute 250. mL of 0.600-M K2Cr2O7 to 300. mL. (b) Add 50.0 mL water to 250. mL of 0.250-M K2Cr2O7. (c) Dilute 125 mL of 1.00-M K2Cr2O7 to 300. mL. (d) Add 30.0 mL of 1.50-M K2Cr2O7 to 270. mL of water.
Write a step-by-step procedure for this serial dilution. The stock concentration of the blue dye standard is 1.00 mg/mL. Serial dilution series from the stock standard with the following final concentrations: 500 ug/mL, 250 ug/mL, 125 ug/mL, 62.5 ug/mL. and a blank that is 0ug/ml
Exactly 10.00-mL aliquots of a solution containing phenobarbital were measured into 50.00-mL volumetric flasks and made basic with KOH. The following volumes of a standard solution of phenobarbital containing 2.000μg/mL of phenobarbital were then introduced into each flask and the mixture was diluted to volume: 0.000, 0.500, 1.00, 1.50, 2.00 mL. The fluorescence of each of these solutions was measured with a fluorimeter, which gave values of 3.26, 4.80, 6.41, 8.02, 9.56, respectively. a. plot the data. b. derive a least squares equation for the data plotted in (a). c. find the concentration of phenobarbital from the equation in (b). d. calculate a standard deviation for the concentration obtained in (c).
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Beaker 0.00200 M Fe(NO3)3, mL 0.00200 M NaSCN, mL total volume, mL 1 3.000 2.000 10.00 2 3.000 3.000 10.00 3 3.000 4.000 10.00 4 3.000 5.000 10.00 5 (blank) 3.000 0.000 10.00 In solutions 1-4 you are adding 3.00ml of 0.00200M Fe3+ to some SCN- solution and dilutiing to a final volume of 10.00ml. What is the new concentration of the Fe3+ due to dilution? Your answer should have 3 sig figs.
You are preparing a 100.0 mL standard solution needed for titration analysis. The NaOH (40 g/mol) pellets are measured to be 0.5341 g in a balance with acceptable uncertainty of +0.001 g. Mass of the pellets is recorded after taring the container. The pellets are put in a beaker and added with 50 ml distilled water measured by a graduated cylinder. The solution is then quantitatively transferred to a 100-mL volumetric flask with uncertainty of ±0.08 mL and diluted to a 100-mL mark. What is the concentration (M) of the solution and estimate its uncertainty by propagation? Assumption: There is no uncertainty in molar mass. Express your answer as C+/- u M.
A stock solution whose concentration is 25.00 mg/mL was provided. A primary standard solution was then prepared by transferring 5.00 mL of the stock solution into a 50.00 mL volumetric flask. The primary standard solution was used to prepare three standard solutions of different concentrations by taking different volumes of aliquots into three 25.00 mL volumetric flasks: Standard Solution #1 #2 #3 Amount of Primary Solution (mL) 5.00 10.00 15.00 Three 25.00 mL volumetric flasks were then diluted to mark with water. Questions #1 What is the concentration of the primary standard solutions, expressed in units of mg/ml? #2 Re-write the concentration of the primary standard solutions obtained from Q#1 in units of μg/mL. #3 Determine the concentrations of three standard solutions in units of µg/mL. #4 What is a requirement for a "blank" solution used in calibration in spectrophotometry? Page 5 (Experiment #3)
You want to make a solution of the primary standard sodium oxalate. It has a molecular mass of 133.999 g/mol (no error). Your analytical balance has an error of 0.2 mg. You add some weighing paper to the balance and it reads a mass of 0.23626 g. You then add sodium oxalate onto the weighing paper until the mass registers as 2.8539 g. You carefully transfer this to a 250.0 ml volumetric flask with an error of 0.1 ml and dissolve the sodium oxalate in a sufficient volume of water. What is the concentration of the oxalic acid (in M) and what is the absolute error (also in M).
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A student creates a calibration plot for serial dilutions of Cu(NO3)2. A graph of concentration in M (x-axis) versus absorbance (y-axis) gives a linear trendline of y = 0.345x - 0.11. e.) Copper(II) nitrate has a solubility of 83.5 g in 100 g of water. Calculate the percent error in the student's concentration.
We prepared a 100.0 μg/mL quinine stock solution by transferring 100.0 mg of quinine to a 1-L volumetric flask, adding 50 mL of 1 M sulfuric acid and diluting to volume with deionized or distilled water. Find the molecular weight of quinine and determine the molar concentration of the 1µg/mL quinine standard.