With reference to the following calibration curve, what is the concentration (M) of a solution with absorbance of 0.3? Abs 0.5 0.4 0.3 0.2 0.1 0.2 0.4 0.6 Concentration (M) A 0.2 M В 0.3 M C 0.4 M D 0.5 M
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- The absorbance values at 250nm of 5 standard solutions, and sample solution of a drug are given below: Conc. (ug/ml) A 250 nm10 0.16820. 0.32930 0.50840. 0.66050 0.846Sample. 0.661Calculate the concentration of the sampleA solution is prepared by diluting 2.79 mL of the blue dye stock solution to 25.00 mL. The measured absorbance for the prepared solution is: Blue dye stock solution = 0.293 M Absorbance at 630 nm = 0.00265 Calibration curve y = 0.0833x A.) What is the theoretical molar concentration? B.) What is the experimental molar concentration? C.) What is the percent error?.Q1. To determine the concentrations (mol/L) of Co(NO3)2 (A) and Cr(NO3)3(B) in an unknown sample, the following representative absorbance data wereobtained.A (mol/L) B (mol/L) 510nm 575nm5×10−1 0 0.714 0.0970 6×10−2 0.298 0.757Unknown Unknown 0.671 0.330Measurements were made in 1.0 cm glass cells.i. Calculate the four molar absorptivities: ∈A(510), ∈A(575), ∈B(510) and ∈B(575).ii. Calculate the molarities of the two salts A and B in the unknown.
- 5. (1) (ii) Ibuprofen (206.3 g/mol) absorbs at 255 nm and shows a molar absorptivity of 3010 L/mol.cm. A tablet of ibuprofen, 0.6654 g, was ground and 0.2203 g powder was dissolved in 20% methanol in a 100 mL volumetric flask (Solution A). Exactly 5.0 mL of Solution A was diluted in a 50.0 mL flask and the resulting solution showed an absorbance of 0.1228 AU in a 1.00 cm cell. (i) What is the concentration (mol/L) of ibuprofen in the 100 mL stock solution? (ii) Report the amount of ibuprofen in mg/Tablet. Ibuprofen H3C | HCCH; 1 H3C CH3 -CH-COOHYellow Dye Calibration Curve y = 254.78x - 0.1414 R? = 0.9792 2.5 1.5 1 0.5 0.002 0.004 0.006 0.008 0.01 0.012 Concentration(M) 3. 2. AbsorbanceWhat is the concentrations in mg/100 ml of the Carbimazole solution of drugs, a(1%, 1 cm) value=(600) at 291 nm, the absorbance at 291 nm was (1.2) ? a. 0.02 b. 2 c. 2000 d. 0.002
- A solution of Tryptophan has an absorbance at 280 nm of 0.54 in a 0.5 cm length cuvette. Given the absorbance coefficient of trp is 6.4 x 103 Lmol-1cm-1. What is the concentration of solution? The answers are as follows A)0.17x10-3mol/L B)2.57x10-3mol/L C)0.17x10+3mol/L D)2.57x10+3mol/LSuppose you measure the absorbance of a yellow dye solution in a 1.00 cm cuvette (b-1.00 cm). The absorbance of the solution at 427 nm is 0.43. If the molar absorptivity of yellow dye at 427 nm is 27400 M¹cm, what is the concentration of the solution in M? Type answer: IA solution of Tryptophan has an absorbance at 280 nm of 0.54 in a 0.5 cm length cuvette. Given the absorbance coefficient of trp is 6.4 x 103 Lmol-1cm-1. What is the concentration of solution? The options are as follows A) 0.17 x10-3 mol/L B)2.56x10-3mol/L C)0.17x10+3mol/L D)2.56x10+3mol/L
- 2. Add the absorbance of your Part III solution to the graph. Llse the of best fit of your qraph to calcuate its line concentration. Concentraton vs Absorbance 1.6 con c: 0.003M 1.4 1.2 0.8 0.6 0.4 0.2 0.0005 0.001 0.0015 0.002 0.0025 0.003 0.0035 Concentration Co.003M) Concentration you have calculated in 2 is CCrO, of Ag CrO4. calculate your experimental ksp of Ag CrO4. = [Cr O4]? Use it to 2. %3D ksp of AgCr Oy = CAg+] [CrO4] Absorbance te OutTube #: 2 3 4 mL 0.0120 M Pb(NO,), 5.00 5.00 5.00 5.00 2.00 3.00 4.00 5.00 mL 0.0300 M KI mL 0.20 M KNO, 3.00 2.00 1.00 0.00 Total vol. (mL) 10.00 10.00 10.00 10.00 Absorbance of soln. 0.381 0.420 0.470 0.542 [I] at equilibrium (M)Table 1.0 of data showing calibration standards prepared by dilution and the absorbance Concentration CuSO4 (M) Volume of 0.5M (ml) Volume of D/Water (ml) Absorbance 0.00 0.10 0.20 0.30 0.40 0.50 II. 0.00 1.00 2.00 3.00 4.00 5.00 5.00 4.00 3.00 2.00 1.00 0.00 1. Construct a graph of concentration against absorbance, clearly show a best fit line. Find regression of your graph. Show how 0.000 0.406 0.638 0.854 1.202 1.276