Why is control of column and detector temperature more important for non- suppressed IC (Ion Chromatography) than it is for suppressed IC?
Q: atalyze cleavage of molecules driven by dition of water talyze transfer of functional groups within…
A: An enzyme is usually a protein that increases the rate of the reaction without itself being used up…
Q: For the structures below, please indicate whether the compound is reducing or non-reducing, and…
A: Carbohydrates that have a free anomeric hydroxyl group can reduce cupric ions to cuprous ions. Such…
Q: 2. Consider the peptide Trp-Arg-Glu-Cys-Gly-Tyr. For the drawings requested below, please show in…
A: Recall that:Amino acid sequences are written with N-terminal amino acid on the left and C-terminal…
Q: The figure to the right shows the setting displays of three pipettes, as labeled. What volume in…
A: Micropipettes are indispensable tools in molecular biology and biochemistry. We cant imagine science…
Q: Draw dipeptide Arg-Thr at pH 6.0. Circle the six atoms that are restricted in one plane. Calculate…
A: Dipeptide is simply 2 amino acids bonded to each other via a peptide bond. The two amino acids that…
Q: Protein aldolase cytochrome c pyruvate kinase lactate dehydrogenase ornithine decarboxylase…
A: In isoelectric focusing, protein separation is based on the isoelectric point (pI). pI is the pH…
Q: Part B Calculate the nonequilibrium concentration of ethanol in yeast cells, if AG = -38.3 kJ/mol…
A: The free energy of a reaction is represented by ∆G. Under standard conditions, it is represented as…
Q: Illustrate the ATP hydrolysis reaction, its regeneration and the AG for both reactions. Show where…
A: ATP is the energy currency of the cell. It is simply an adenosine molecule bonded to a triphosphate.…
Q: The hydrolysis of pyrophosphate to orthophosphate drives biosynthetic reactions such as DNA…
A: Enzymes are proteinaceous entities involved in chemical reactions contributing to the precedence of…
Q: Many biochemists go bananas, and justifiably, when they see a Michaelis-Menten plot like the one…
A: For a one-substrate enzyme catalyzed reaction, the Michaelis-Menton equation shows the quantitative…
Q: Select the graph that correctly illustrates the effect of a negative modifier (effector) on the…
A: Negative modifiers are species that decrease the activity of enzymes. This leads to a decrease in…
Q: Thr-Lys-Pro-Ile-Val-Ala-Pro-Met-Glu-Tyr-Gly-Lys Write the sequence using one-letter abbreviations.…
A: Amino acid sequences are written with N-terminal amino acid on the left and C-terminal amino acid on…
Q: The ESI-MS spectrum in positive ionization mode for lysozyme is obtained. a. What is the molecular…
A: The y-axis of the ESI-MS spectrum reflects relative abundance and the x-axis the m/z (mass charge)…
Q: A patient is suspected of having low stomach acid, a condition known as hypochloridia. To determine…
A: Acids and bases are two chemical substances that react with each other to form a salt and water.…
Q: You have isolated a protein from the bacterium E. coli and seek to confirm its identity by trypsin…
A: Mass spectrometry is an analytical gel-free proteomics method used for identification of proteins,…
Q: H expresses the molar concentration of hydronium ions in an queous solution on a logarithmic scale.…
A: The pH :- pH expresses the molar concentration of hydronium ions in an aqueous solution on a…
Q: 0.5 M NaOH. Write the acid base reaction that is taking place and use an ICE table to show how the…
A: Number of moles in 100mL of 5M acetic acid can be calculated by the formulaVolume in L ×…
Q: | a) Match the type of bond with the role below: Bond_type (a) phosphodiester (b) N-glycosidic (c)…
A: Nucleic acids are one of the 4 biomacromolecules. Nucleic acids are generally found in 2 forms in…
Q: A lysine is on the surface of a protein near an arginine, how does this alter pKa of lysine
A: The pKa of an amino acid's side chain determines whether it is protonated (+) or deprotonated (-)…
Q: Consider the following isomerization reactions of some simple sugars and values for their standard…
A: Details
Q: The pH of an aqueous solution containing fermentation nutrients with low buffering capacity should…
A: Fermentation is a critical process in biotechnology and the food industry that involves microbes…
Q: What is the total mass of oxygen in a single molecule of glucose? Express your answer in amu to 5…
A: Glucose is a carbohydrate, a monosaccharide. In most living cells, glucose is oxidised completely…
Q: Using the pka data for lysine, draw the molecular species present at each of the following pH values…
A: Lysine has 3 ionizable groups in it. They are;alpha- carboxyl groupalpha-amino groupside chain basic…
Q: In an ammonia molecule, one nitrogen atom (atomic number = 7; 1s² 2s²2p³) forms covalent bonds with…
A: Ammonia (NH3) is a colourless gas with a strong odour that is made up of one nitrogen atom coupled…
Q: (a) Using the lecture entitled “On the Origin of life” by Jack Szostak. In one paragraph, summarize…
A: In his lecture titled "On the Origin of Life," Jack Szostak delves into the concept of an "RNA…
Q: Draw the amino acids serine and threomine bonded together with a petide bond.
A: Amino acid
Q: Substance X is transported by passive transport and Substance Y is transported by active transport…
A: Membrane transport refers to the movement of solute substances across or through a membranous…
Q: A researcher isolates a new protein and finds that it contains 0.755% serine by weight upon amino…
A: A peptide is a short chain of amino acid residues linked together via a peptide bond. The peptide…
Q: The value of AGO for the conversion of 3-phosphoglycerate to 2-phosphoglycerate (2PG) is +4.40…
A:
Q: The reagent dithiothreitol (DTT) is commonly used to reduce disulfide bonds in Proteins. The…
A: DTT works as a reducing agent by donating electrons to break disulfide bonds in proteins, leading to…
Q: 1: QAMGRAGDLKYLGLHSV Peptide 2: ALMALFMVMALVLVSVLFIA Peptide 3: MVEDLLKQIARYLISE (a) Circle all of…
A: We are authorized to provide three sub parts at a time; since you have not mentioned which part, we…
Q: Substrate concentration how reaction rate (velocity) varies with substrate concentration. Rate…
A: Enzyme is usually a protein that catalyzes a reaction by increasing the rate or the velocity of the…
Q: The pK₂ of the x-carboxyl group of serine is 2.21, and the pKa of its a-amino group is 9.15.…
A: pKa is the pH at which a weak acid is 50% dissociated. % ionisation of a weak acid = % ionisation of…
Q: How would the following enzymes speed up or slow down glycolysis? - high levels of Amp - high levels…
A: In glycolysis, a 6-carbon molecule of glucose-6-phosphate is broken down into 3-carbon pyruvate. It…
Q: Which of the above curves corresponds to myoglobin in a solution containing physiological…
A: The given curve is an oxygen dissociation curve. The curve depicts the relationship between the…
Q: Decide whether each of the following statements is true about ornithine carbamoyltransferase. It's a…
A: Ornithine carbamoyltransferase is an enzyme is important in the urea cycle. Urea cycle is a…
Q: to use the pH simulator to answer the question: . Select the Macro setting on the simulator…
A: Go to the pH simulator homepageOpt for the "Macro" mode on the simulator.Find the green pH meter and…
Q: 19. Draw all 20 of the genetically encoded amino acids at (a) pH = 1; (b) pH = 1; (c) pH = 12.…
A: Amino acids are organic compounds that form the building blocks of proteins in cells. The structure…
Q: Two possible point mutations are the substitution of lysine for leucine or the substitution of…
A: Amino acid mutations refer to changes in the DNA sequence that result in alterations to the sequence…
Q: be the A form of DNA, the B form of DNA, the Z form of DNA, or all three. A form the favored form of…
A: DNA called deoxyribonucleic acid is made up of nucleotides. Each nucleotide is nade up of a -…
Q: Explain why unfolding or aggrrgation may occur using changes in entropy and enthalpy of the protein…
A: Unfolding or aggregation of proteins can occur due to changes in entropy and enthalpy, which are…
Q: What are the characteristics of a secondary antibody used in Western blotting? contains a covalently…
A: Western blotting is a laboratory technique used to detect a specific antigen containing protein in…
Q: After a polypeptide chain has been synthesized, certain amino acids in the peptide may become…
A: The modified amino acid shown in the structure you provided is serine (Ser).The modification that…
Q: had a response from a peer and this is what they said: You mentioned that aliphatic amino acids…
A: As per the central dogma of molecular biology, DNA contains the code that is necessary for the…
Q: What if C-1 of the oxaloacetate were labeled instead, when would the label be released as CO2?
A: Oxaloacetate is a four-carbon compound, and carbon-1 (C-1) refers to the first carbon atom in the…
Q: V, (μmol-min-¹) 0.35 1/V₂ 0.3 0.25 0.2 0.15 0.1 0.05 0 0 50 100 150 200 250 300 350 400 450 [S] (nm)…
A: Michaelis Menten kinetics and Lineweaver Burk plot show the activity of the enzyme catalyzed…
Q: What is the difference in atp formation in glycolysis and o inactive metabolism
A: Glycolysis is a metabolic pathway that occurs in the cytoplasm of cells. It breaks down one molecule…
Q: 7. Draw a diagram of the heme complexes in deoxyhemoglobin and oxyhemoglobin (complete porphyrin…
A: A heme complex is a critical molecular structure found in various biological molecules, such as…
Q: A weak acid, HA, has a pK₁ of 4.756. If a solution of this acid has a pH of 4.101, what percentage…
A: To calculate the percentage of the weak acid HA that is not ionized in a solution with a given pH,…
Q: A different Fab fragment binds to lysozyme with a dissociation constant of Ka = 10-6 M. A 1 nM (10-9…
A: Ligand receptor complex is a term used to describe the non-covalent interaction between a ligand and…
Why is control of column and detector temperature more important for non- suppressed IC (Ion Chromatography) than it is for suppressed IC?
Step by step
Solved in 4 steps
- Why can't we determine Vo, Ve and Vt for affinity chromatography?Name two radioisotopes used within the medical imaging processes, one administered by inhalation and one used by intravenous injection. For both of these radioisotopes, what are their half-life and what is the relevance of that to why it is used? What are both of these specific use in diagnostic imaging?What are they treating?The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?
- What is Dry Flash Column Chromatography? Please explain at your own words( around 500 words).For the same chromatographic column and experimental conditions Rs when H = 3 cm = Rs when H = 5 cm Rs when H = 3 cm > Rs when H = 5 cm Rs when H = 3 cm < Rs when H = 5 cm The two quantities cannot be compared due to insufficient information.For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What are the initial concentrations used for tubes 3, 5, and 6? Show pertinent solutions. What are the dilutions of the last positive tube and first negative tube respectively? Show computation.
- The figure above depicts an agar cube with a side length of 13\, \text{mm}13mm13, start text, m, m, end text. In an experiment, students submerged the cube in red dye for 121212 hours. The red dye permeated 1\, \text{mm}1mm1, start text, m, m, end text on each side, as indicated by the shading in the figure. Volume of a rectangular solid: V = lwhV=lwhV, equals, l, w, h Calculate the volume of the agar cube that remained unpenetrated by the red dye.The BSA stock solution from the previous problem was then diluted to generate a set of standard solutions of known concentrations. After performing biuret assay on these solutions, their absorbance at 540 nm were measured using a UV-vis spectrophotometer. The following data were obtained. Concetration of BSA Absorbance (mg/mL) 0.1 0.048 0.2 0.095 0.4 0.191 0.6 0.290 0.8 0.380 1.0 0.485 Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an unknown sample having an absorbance of 0.325.(b) Table Q1 below provides optical measurements of samples with known concentrations of analyte (ten samples were measured and the average and standard deviation are presented). They are also represented graphically in Figure Q1, below the table. Analyte concentration (nM) 0.1 0.2 0.5 1 2 5 10 15 fluorescence intensity 300000 250000 200000 150000 100000 50000 0 0 Table Q1 Fluorescence intensity (average) 9300 9200 10800 19600 37200 90000 178000 224000 5 [Analyte]/nM 10 Standard deviation 2000 1200 1000 2000 10000 30000 25000 40000 15 Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard deviation. (ii) Calculate the limit of detection (LOD) for this sensor. (iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a signal of 142800. The coefficient of variation of the device is 5%. Explain whether the diagnostic lab would have the confidence to report that the sample has a concentration in analyte of 8 nM or…
- Why is UV light used in Column chromatography experiment ?The usual dose of digoxin for rapid digitalization is a total of 1.0mg, divide into two or more portion at intervals of 6 to 8 hours. How many milliliters of digoxin elixir containing 50mcg/mL would provide this dose?In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.