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- Given the electrophoresis profile of a Sanger sequencing result, what was the sequence of the original DNA sample used for sequencing? GGTAACC CCAATGG GGTTACC CCATTGGacconline.austincc.edu/ultra/courses/_891351_1/cl/outline O gel electrophoresis O PCR O genomic library DNA microarray A patient is suspected of having a certain type of cancer that involves several genes. Doctors want to determine whether this patient is expressing these cancer genes and to what leve each gene is being expressed. What technique may be used? O molecular cloning O CRISPR gene editing 5 genetic recombination that occurs naturally (think horizontal gene transfer, meioșis, fertilization, or mutations). Ar : Y EV АУ ΩΘΙ 用く X A MacBook Air & 8 88 Q V Ix % 0 8A EXE D B 图 Q 用图 5 d Show All † 19 EHR ImmunizaTRANSCRIBE this DNA sequence: TACGTTACT AUGCAAUGA O ATGCAATGA AUGGATUGA TACGTTACT
- What is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis profile from the Sanger sequencing method? ddATP ddCTP ddGTP ddTTPSecond Base Analyze the following DNA sequences: A UUU UCU UAU UGU Phe Tyr UUC UCC UAC UGC Original: AGAGAGAGAGAGAGAGAG Ser UUA UCA UAA Stop UGA Stop Leu UUG UCG UAG Stop UGG Trp CUU CCU CAU CGU His Mutated: AGAAGAGAGATCGAGAGA CUC CC CAC CGC Leu Pro Arg CỦA CCA CAA CGA Gin CUG CCG CAG CGG AUU ACU AAU AGU Ser Asn What amino acid sequence will be translated based on the AUC Ile ACC AAC AGC The mutated DNA? Hint* must transcribe and translate the AUA ACA AGA AAA arg Lys Met or Start AUG ACG AAG AGG mutated strand to find the answer. GUU GCU GAU GGU Asp GUC GCC GAC GGC Val Ala Gly G GUA GCA GAA GGA Glu GUG GCG GAG GG First Base Third BaseSample Gel Electrophoresis: A brother and sister's DNA are cut with the same restriction enzyme and then the resulting DNA fragments are run on a gel next to each other. DRAW THE BANDS on the gel where theywould appear: • Carissa's DNA was cut into 4 pieces: 2- The pieces were 20 base pairs long, 10bp long, 8 and 5. 4- • Christopher's DNA was cut into 5 pieces: 6- They were 25 bp long, 10, 8 and the last two pieces were 8- koth 2 be long. 10 You can make a scale to helo you > 12 14 13. What did you do about the 2 DNA fragments that were 16- 18- both 5 base pairs long? 20- 22 24- 26 14. How many DNA fragment sizes do these kids have in common? Both the kids have two fragments similar. 8bp & 10bp I O 00 O 00 Carissa Smith Christopher Smith
- BamHI KpnI SpeI XhoI PatI HindIII 400 500 200 300 700 NotI 75 2580 ECORI Frog DNA BamHI 575 KpnI HindIII 625 2150 HindIII 700 PstI clal 750 РКАВОО 2700bp 915 1900 SpeI AluI 1050 1525 BamHI ori You wish to make a recombinant DNA molecule that will contain one piece of pKABOO vector DNA and one piece of frog DNA so that you can clone a segment of frog DNA. You want cells containing your recombinant plasmid to be amp' and tet and you want to use enzymes that cut within the insertional marker gene. (Note that tet means that there is no functional tet gene in the plasmid.) Be sure that your plasmid has the ability to replicate autonomously in a bacterial cell. You do not have to include the entire frog DNA given below in your recombinant plasmid. Restriction enzymes would be used to clone segment of frog DNA The size of the recombinant plasmid is bp. The recombinant plasmid when transformed into E. coli confers resistance to which of the following antibiotics: Oampicillin only Otetracycline…PCR primers Below is a 300 base pair fragment of DNA. The top strand is written in the 5' to 3' direction. The bottom strand is written 3' to 5'. There are also two primer sequences; both primers are written 5' to 3'. Note that we are displaying a double-stranded DNA fragment, but primers will only bind to one of the two displayed strands. 5' ACCOȚAGCTATATOCTATCOTGACOTATCOGCOCATTAAȚCGGGATCGAT 3 3' TGGCATCGATATACGATAGCACTGCATAGCCGCGTAATTAGCCCTAGCTẢ 5 50 5' AGCTCGCTAGCAGGAGAGATATCGCTCATAGCTCCGATCGATGCCGCTAA 3 100 3' TCGAGCGATCGTCCICTCTATAGCGAGTAICGAGGCTAGCTACGGCGATİ 5' 5' TATAGCTCTCTGCGGATATÇGCATẠTACCAAGGCCCTACGTATGTAGCTA 3 150 3' ATATČGAGAGACGCCTATAGCGTATATGGÍTCCGGGATGČATACATCGAŤ 5 5' TGCGȚATATÇGGAGAGTCCTGGATAT GGAGCTTGACTGCAGAGAGCTCGA 3 200 3' ACGCATATAGCCTCICAGGACCTATACCTCGAACÍGACGICTCTCGAGCİ 5' 5' TATGCGCTTAGGCCGTATATGCTTGGGGAAAGCTCTATGTATGCTATGTG 3 250 3' ATACGCGAATCCGGCATATACGAACCCCTITCGAĞATACATACG ẢTACAČ 5' 5' TGCATOTGCTATOCAACGTTC GGATTGCGȚAGCAGTAATAGCGCCGATTO 3' 300 3'…What is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis profile from the Sanger sequencing method?
- Can you explain thie each of the statement given i dont really understand the dna recombinant is used as a molecular cloning and application for recombinant dnaWhat is the relationship between fluorescence intensity of a spot and the amount of DNA in a sample for a Virochip DNA microassay? From the following which is the best choice Less DNA results in greater fluorescene since the laser passes through he sample eaiser More DNA equals to more intense fluorescence since the DNA makes a layer to refelct the laser light More DNA equals to more intense fluorescence since of more dye on the DNA is fluorescene More DNA results in to more fluorescence since there's more DNA to be excted by the laser None of the aboveWhich of these is not a tool for comparing DNA sequences? BLAST Fasta PLINK A dotplot e.g. dotlet