Which of the following is NOT true about the production of monoclonal antibodies? what option below is the answer? Monoclonal antibodies can be used in diagnostic lab tests Hybrid cells proliferate into hybridomas that grow continuously in culture to produce antibodies Spleen cells hybridize with myeloma cells (cancerous B cells) in culture Spleen cells are harvested from mice that were immunized with an antigen Each hybridoma clone produces antibodies against several different antigens

Which of the following is NOT true about the production of monoclonal antibodies? what option below is the answer? Monoclonal antibodies can be used in diagnostic lab tests Hybrid cells proliferate into hybridomas that grow continuously in culture to produce antibodies Spleen cells hybridize with myeloma cells (cancerous B cells) in culture Spleen cells are harvested from mice that were immunized with an antigen Each hybridoma clone produces antibodies against several different antigens

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter42: The Immune System

Section: Chapter Questions

Problem 1VCQ: Figure 42.11 Which of the following statements about T cells is false? Helper T cells release...

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All of the following are true about neutralizing antibodies, except: Group of answer choices Bind to the spike protein Bind to the ACE2 receptor binding domain Prevents virus from replicating in cells Have emergency approval use for use as treatment in COVID19 Allows viruses to enter cells and replicate
High dose hook effect occurs when the sperm cells are hook shaped the mobile antibody-antigen complex binds to the stationary antibodies not enough sample has been added the mobile antibody-antigen complex binds to excess antigens excess antigens prevents the mobile antibody-antigen complex from binding to the stationary antibodies
You are interested in performing indirect immunofluorescence light microscopy to observe the localization of the catalase enzyme in the cultured HeLa cells, obtained historically from the cervical tumor of Henrietta Lacks. You were going through the lab stock and found a few primary and secondary antibodies. Which of the following secondary antibody can you use in your experiments? O All of the mentioned antibodies can be used in the experiment Goat anti-human antibody conjugated to 10 nm gold Goat-anti-human catalase conjugated to 10 nm gold O Human anti-catalase antibody conjugated to fluorescent rhodamine Goat anti-human antibody conjugated to fluorescent rhodamine
The antibody surface involved in antigen binding varies depending on the size and nature of the antigen. This surface can be concave or flat, and sometimes, can have extended protrusions. This is accomplished by: Flexibility in the hinge regions of the antibody allowing rotation of the antigen-binding sites Some antibodies using V region framework sequences instead of the CDRs to bind antigen The ability of different CDR sequences to form many structurally distinct shapes and surfaces The ability of the same heavy chain to pair with different light chains The differential usage of κ versus λ light chains, as κ chains form concave
hinge region Fc Fab kappa alpha chain Drag answer here Drag answer here Drag answer here Drag answer here Drag answer here provides flexibility to permit binding to different antigenic arrangements a heavy-chain isotype a light-chain isotype the arms of the antibody that bind antigen the stem that carries out effector function of antibodies through interaction with cell-bound receptors and serum proteins
The secondary antibody used in the laboratory for western blotting is conjugated to which enzyme? alkaline phosphate horseradish peroxide GAPDH alkaline decarboxylase
What are the characteristics of a secondary antibody used in Western blotting? contains a covalently attached tag recognizes the Fc region of the primary antibody recognizes the Fab region of the primary antibody recognizes antibodies from multiple species Incorrect
What scenario best describes an autoimmune reaction? Group of answer choices The destruction of donated red blood cells by the recipient’s immune system The production of antibodies against Streptococcus pyogenes antigen resulting in cross-reaction against an antigen found on heart valves The activation of Mast cells by pollen The production of antibodies against Streptococcus pyogenes resulting in the deposit of antigen-antibodies in the kidney leading to glomerulonephritis
In immunofluorescence experiments, what is the role of the primary and secondary antibodies? (select all that applies) Group of answer choices The primary antibody binds the protein of interest whereas the secondary antibody binds the primary The primary and secondary antibodies can be interchangeable The secondary antibody is labeled with a fluorophore and is therefore responsible for generating the signal The secondary antibody is less important and therefore the experiment can be performed without it
The indirect immunofluorescence ("fluorescent Ab") test shown below is performed to test for: + AA T. pallidum from Specific antibodies laboratory stock in serum of patient Antibodies bind to T. pallidum + ㅅ Fluorescent dye-labeled anti-human immune serum globulin (This will react with any immunoglobulin) Da Fluorescent spirochetes (see Figure 3.6b) None of the other four answers are correct The presence of T. pallidum bacteria in the patient's blood Whether the patient has made antibodies against other human antibodies Immunologic evidence of current or past infection with T. pallidum (i.e., Ab against T. pallidum) Growth of T. pallidum in culture from a syphilis patient
Drag each label to the appropriate box to indicate if the label is associated with B cells or T cells. Labels can be used more than once. Formation of clone when activated Production of antibodies Cellular immunity Stimulated by cytokines B Cells Humoral immunity Targets cancer cells and virally infected cells Interact with antigen- presenting cells Formation of memory cells T Cells
You can choose one or more than one option The antibody region that bind the antigen region with a high affinity is called: complementary determining region (CDR) hinge region heavy chain hypervariable region of the variable domain epitope