Which of the following is incorrect about the Michaelis-Menten plot? a. The y-axis is velocity and the x-axis is [substrate] Ob. It is based on E + S to reversibly form ES and then this to form E + P C. The data is plotted using the Michaelis-Menten equation Od. It has a sigmoidal shape
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![Which of the following is incorrect about the Michaelis-Menten plot?
a. The y-axis is velocity and the x-axis is [substrate]
b.
It is based on E + S to reversibly form ES and then this to form E + P
O c. The data is plotted using the Michaelis-Menten equation
Od. It has a sigmoidal shape](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa7715250-a16c-4a08-bd4e-10bedd823d58%2F802e9ef8-6203-4497-89b2-a57847656770%2F2ckz8ika_processed.jpeg&w=3840&q=75)
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- You want to prepare 500 mL of the macronutrient and micronutrient stock solutions which have 100X and 1000X higher concentration, respectively, than the required concentration in the culture mediuma)If 0.17 g/L KH2PO4 is required in MS medium, determine the mass (g) of KH2PO4 that is required to prepare the stock solution and the volume required (mL) to prepare 200 mL MS medium. Show your work.As shown in the image below, 10 µl of a stock solution (tube #1) are serially diluted into 0.990 ml of buffer. a. What is the dilution factor for this serial dilution? b. If the concentration of the stock solution in tube #1 is 0.8 M, what is the concentration of the solution in tube #3? 10 ul 10 l 10 0.990 ml SM buffer 0.990 ml SM buffer 0.990 ml SM buffer Tube #1 tube #2 tube #3 tube #4 For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac).Please identify the following on the Michaelis- Menten plot shown A, B, C, and the X and Y axes. Please briefly explain the significance of each. A B с
- Match the following formulas with their correct descriptions form the drop down menu provided. Formulas 1. D subscript p equals N over A 2. D subscript p equals N over V 3. increment N equals open parentheses N plus I close parentheses minus open parentheses M plus E close parentheses 4. g r equals fraction numerator increment N over denominator increment t end fraction 5. c g r equals fraction numerator increment N over denominator N end fraction Descriptions: Used to calculate the rate of change in the population in relation to its initial population number Answer Used to calculate the change in population size Answer Used to calculate the number of individuals occupying a volume of air or water Answer Used to calculate the change in the number of individuals in a given population over a period of time. Answer Use to calculate the number of individuals occupying a land area AnswerPhenylephrine and nafazolin solutions are used as decongestants to relieve nasal congestion. Your patient has been given the following prescription below. You need to prepare 100 g of this nasal spray and make it isotonic. 1. There is an available 25% w/w Phenylephrine solution in the pharmacy. How many g of this solution will you need to supply the needed 0.5% of the patient? A. 1.0 g В. 1.5 g С. 2.0 g D. 4.0 g 2. Given the amount calculated on the previous item, and if the E value of phenylephrine HCl is 0.32, calculate its corresponding sodium chloride tonicic equivalent. А. 0.16 B. 0.64 С. 1.60 D. 6.40 3. If the E value of nafazolin is 0.27, what is the sodium chloride tonicic equivalent of the patient's needed nafazolin? A. 0.0135 B. 0.1350 1.3500 D. 13.500 4. What is the combined sodium chloride tonicic equivalent of the remaining ingredients (edetate disodium (E =0.24) and sodium metabisulfite (E = 0.7)? A. 1.060 B. 0.106 С. 1.290 D. 0.129 5. To make the solution isotonic, what…A flat (not spherical) tissue engineered skin (thickness 2.5 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.4 E-9 m2/s, and according to experiments that you’ve performed, you want to make sure that the concentration of oxygen in the device doesn’t fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5.9 E-17 mol/[cell◦s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?
- You have an order for 1 gram of Cefazolin in D5W 100 ml. You have added 5 ml of sterile water to the 1 gram vial to reconstitute powder. However the recommended manufacturer’s diluent amount is 10 ml of sterile water for a final concentration of 100 mg/ml. How would reconstituting the vial with 5 mls affect the concentration and the final calculated dose. Please answer with explanation ASAP. I will really upvote. ThanksA flat (not spherical) tissue engineered skin (thickness 3.9 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.2 E-9 m?/s, and according to experiments that you've performed, you want to make sure that the concentration of oxygen in the device doesn't fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5 E-17 mol/[cell•s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?You are given a 27 % (w/v) solution of psicose. Molar mass: psicose (C6H12O6) = 180.156 g/mol. ou have a 0.75 mg/mL solution of bovine serum albumin (BSA). You add 1 mL of the 0.75 mg/mL BSA solution into a standard cuvette (1 cm path length), set your spectrophotometer to 280 nm and read the absorbance of this BSA solution. Molecular mass of bovine serum albumin = 66.4 kDa. Molar extinction coefficient (e) of BSA = 43824 M-1 cm-1 (at 280 nm). What absorbance reading will you obtain?
- Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.The Lineweaver-Buck plot is said to give undue weight to measurements made at low substrate concentrations, when the results are likely to be more accurate?You put 50 uL of 0.1 mg of protein into a 1 cm pathlength cuvette containing 3 mL of LDH reaction mixture and measure a slope of 0.125 abs/min in an LDH assay. What is the reaction rate in uM/min? Show your work.