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- (A) What property of a protein might make it difficult to transfer it from polyacrylamide gel to nitrocellulose? Explain your reasoning. (B) What parameter of the gel transfer protocol can be adjusted that might help improve the transfer of these problematic proteins to the nitrocellulose membrane? Explain your reasoning. (C) How can we check if proteins have been successfully transferred from the polyacrylamide gel to nitrocellulose?List 4 different actiated carrier molecules, for each one, list both the oxided and reduced formsWhich patch clamp technique should be used if you want to investigate the effect a compound has presynaptically with varying concentration?
- What phrases do the CDC and other leading officials now use to describe the risk of contracting COVID-19 through surfaces?How is the protien gradient formed?Electrophoresis is performed at PH 6.8 on a mixture of mutated hemoglobin that differ from normal haemoglobin (Hb) only by the substitution of one amino acid- Hb X: Val replaced par Glu - Hb Y: Asp replaced by Leu - Hb Z: Glu replaced by Lys What will be the order of migration between cathode and anode of these mutated Hb compared to normal Hb? Justify your answer.
- what is the concetration of a lysozyme solution with an absorbance of 0.720 measured at 280 nm( e= 36000 -1M -1cm,l=1cm)How might the technique of affinity chromatography (see animation) be used to purify lectins?Describe the role of Clavulanic acid when it is used in conjunction with Amoxicillin . Give typing answer with explanation and conclusion
- What are the roles of adhesion molecules – CAMs. What five families of protein fit into this category? What are their structures?Amoeboid cells that migrate through our tissues, such as the class of white blood cells known as neutrophils, often do so in a directed manner, triggered, for instance, by chemical signals released by pathogens such as bacteria. Directed migration in response to a chemical stimulus is known as chemotaxis. Part of an efficient chemotactic response is the ability of cells to polarize. As is the case with our structurally-polar polymers like F-actin or microtubules, polarization here refers to an asymmetry in the cells, rather than an electrical charge. In this case, it involves one part of the cell becoming the “front” (or leading edge) and another the rear. In a well-polarized, migrating cell, it’s been observed that an active form of Rac (which, in turn, can activate ARP 2/3) is concentrated towards the front of the cell, whereas an active form of Rho (which, in turn, can activate formin, inhibit ADP, and activate myosin II) is found toward the rear of the cell. Based on your…Amoeboid cells that migrate through our tissues, such as the class of white blood cells known as neutrophils, often do so in a directed manner, triggered, for instance, by chemical signals released by pathogens such as bacteria. Directed migration in response to a chemical stimulus is known as chemotaxis. Part of an efficient chemotactic response is the ability of cells to polarize. As is the case with our structurally-polar polymers like F-actin or microtubules, polarization here refers to an asymmetry in the cells, rather than an electrical charge. In this case, it involves one part of the cell becoming the “front” (or leading edge) and another the rear. In a well-polarized, migrating cell, it’s been observed that an active form of Rac (which, in turn, can activate ARP 2/3) is concentrated towards the front of the cell, whereas an active form of Rho (which, in turn, can activate formin, inhibit cofilin/ADP, and activate myosin II) is found toward the rear of the cell. Based on your…