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Question 32
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- If the TLC plates had been developed in a more polar solvent, would retention factors on the whole be larger, smaller, or unchanged? Briefly (1 - 2 sentences) explain your reasoningAs solvent polarity increases, the Retention Factor (Rf) of compounds on the TLC plate increases. Group of answer choices True Falsewhich would stain with KMnO4 after a TLC Please provide only typed answer solution no handwritten solution needed allowed
- What is the purpose of thin layer chromography lab? What is a hypothesis I can use when conducting a TLC lab and how can that hypothesis be tested?what is the unkown that line up with caffeine and acetaminophen and acetyl salicylic acid in TLC experment it's a drug compounedhow does TLC determine if a sample is pure?
- Plant pigments in spinach were separated with TLC with cellulose as thestationary phase and 80/20 diethyl ether/acetone mixture as themobile phase. If needed, the solvent structures in the lab Appendix.1. Determine the Rf values of each plant component on this TLC plate.2. Arrange the plant components from most polar to most nonpolar. Please answer both 1 and 2 (picture below is the same question attached )If the TLC chromatogram above was duplicated but using a column, draw the set-up and label approximate spots.It is acceptable to draw the TLC spotting line with a pen as long as it is marked very lightly. True False
- During TLC, what is the stationary phase and what is the mobile phase?.Which of the following solvents is not commonly used in reversed-phase HPLC? a) Ethanol Water b) Hexane c) Acetonitrile d) Methanol e) water5. Summarize your conclusions from the TLC simulator. Circle the correct answer in each description about TLC separation of organic compounds on silica gel plates. a) A more polar compound has a lower/higher retention factor. b) A less polar compound has a lower/higher retention factor. c) A more polar solvent causes a lower/higher retention factor. d) A less polar solvent causes a lower/higher retention factor. e) To increase the retention factor of a spot, you should add more ethyl acetate/hexane.
