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Q: Which of the following methods cannot be used to visualize spots on a TLC plate? Group of answer…
A: Which of the following methods cannot be used to visualize spots on a TLC plate? Group of answer…
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Q: TLC.
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Q: 1. You know that these types of compounds can be analyzed by either GC or HPLC but you are told by…
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A: Rf value of each spot = To be determined Identity Of Unknown Solution = To be Determined
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Q: how does TLC determine if a sample is pure?
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Q: We used tab water to extract caffeine from tea instead of using distilled water. How does it affect…
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Q: You are treating the TLC plate with 5% of sulfuric acid in ethanol. Briefly describe how this works.
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Q: does a co spot on TLC plate have two Rf values?
A: co-spot in TLC
What are some ways to visualize a TLC plate? Why may it be necessary to have more than one method to visualize a TLC plate?
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- What is Ultra-Performance Liquid Chromatography (UPLC)? What are the differences and advantages of UPLC over HPLC?Although TLC is qualitative and tells you what is present in a given sample, it can also be used to determine where a compound might be lost: What samples could you test on a single TLC plate that might give you this information?1. In thin layer chromatography (TLC), explain how the pigments separate on a piece of paper. 2. If the spots are colorless on a TLC plate, how can you make it visible? Enumerate 2 ways and discuss each.
- 5. The following TLC plate was developed in 4:1 hexane/acetone. a) Draw what the TLC plate could look like if it were developed in 9:1 hexane/acetone. b) Draw what the TLC plate could look like if it were developed in 1:1 hexane/acetone. 4:1 hexane/acetone 9:1 hexane/acetone 1:1 hexane/acetoneConsider the following molecules. Which statement(s) is TRUE? CH3 CH3 но ČH3 но ČD3 i. You could differentiate between the two with a spot test. ii. You could differentiate between the two with liquid chromatography. iii. You could differentiate between the two with TLC. iv. You could differentiate between the two with a microcrystalline test. O a. i and iv only O b. ii and iii only O c. iii only O d. i only e. Neither i, ii, iii, nor iv are correct statements.A student ran a TLC plate using a 90:10 hexane:ethyl acetate developer and this was the outcome. How can they improve their next trial?
- An example of planar chromatography is: Column chromatography HPLC GLC TLCAside from TLC and paper chromatography, what are the other types of chromatography. Discuss their principles, applications and limitations.How does ammonia, dimethylglyoxime (DMG), and 8-hydroxyquinoline (8HQ) interact with your cations, leading to the evolution of colored species? Context: Paper chromatography experiment (separation of inorganic cations) with a 9:1 acetone/ HCl solvent
- How is TLC used to determine the purity of a sample?4. Enumerate the differences between a serological and a volumetric pipette. 5. Enumerate the uses, advantages, and limitations of a glass versus a plastic volumetric pipette.2. Consider the following example GC Chromatogram from a publication about aggression among Mediterranean Acrobat Ants (C. scutellaris). This particular sample was generated by rinsing hydrocarbons from ant exoskeletons. From the chromatogram, what is the minimum number of unique compunds present in the sample? What data led you to that conclusion? (Don't overthink it) 2 8000000 7000000 6000000 5000000 4000000 3000000 2000000 1000000 Counts (Abundance) 5 19 6 7 0 23.00 24.00 10 | 11 13 17 20 14 15 16 12 teel | 22 19 21 ли 25.00 26.00 27.00 Time "Example of a Gas chromatography-Mass spectrography (GS-MS) chromatogram of hydrocarbon molecules in C. scutellaris" by Filippo Frizzi, et.al, in PLoS One is licensed under CC BY 4.0