Vo (uM/min) Vo (uM/min) [Substrate] (mM) Control (no Inhibitor) +Inhibitor A (250 uM) 0.00 0.00 0.00 1.000 9.89 3.95 2.000 16.34 6.85 3.00 20.86 9.07 4.00 24.22 10.83 5.00 26.80 12.25 Control Inhibitor A X X X
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Find the control Vmax and Km value, and use this to find the Vmax apparent for inhibitor A.
![Vo (uM/min)
[Substrate] (mM) Control (no Inhibitor)
0.00
0.00
1.000
9.89
2.000
16.34
3.00
20.86
4.00
24.22
5.00
26.80
Control
X
X
Vo (uM/min)
+Inhibitor A (250 uM) +
0.00
3.95
6.85
9.07
10.83
12.25
Inhibitor A
X](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fbb3653f4-2dcf-413c-aebd-a6edb6b69637%2F554148cb-da7b-494e-a9ae-d3b17cce43c2%2Fahlvfo6_processed.png&w=3840&q=75)
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- graph the data from the table after taking recipocals of [S], V without inhibitor and V with inhibitor. It should be Lineweaver - Burke. use graph paper or excel. however upload graph and answers as 1 document as jpeg, pdf, or word. there should be 2 lines on 1 graph and both lines should cross the Y axis and hit the X axis (negative X axis). label both axes, determine Km and Vmax (show work) in the presence and absence of inhibitor State type of inhibition and if inhibitor resembles the substrate or not. can more substrate relieve the inhibition? [S] (UM) 3 10 30 5 90 Velocity (umol minute-¹) No inhibitor 10.4 14.5 22.5 33.8 40.5 Inhibitor 4.1 6.4 11.3 22.6 33.8This is a walk-through of Minimum Inhibitory Concentration, MIC: This tray has 8 rows, 4 for Staphylococcus aureus (SA) and 4 for Methicillin-Resistant S. aureus (MRSA). Each row has 12 compartments, or wells. The wells have different concentrations (ug/ml) of the antibiotic Oxacillin in them. From Left to Right, the concentration of Oxacillin is going down acillin concentration, pg/ml 64 32 16 8 4 2 1 0.5 0.25 0.13 0.06 0 SA dn The same amount of S. aureus broth culture is added to each well and the tray is incubated. A color change shows that S. aureus grew in a given well. MRSA What is the lowest concentration at which SA is inhibited (does not grow)? 64mg/ml What is the lowest concentration at which MRSA is inhibited? What is the MIC for Oxacillin vs SA? What is the MIC for Oxacillin vs MRSA? - Notes Comments 33°FDetermine the Ki for the inhibitor at 30 °Cand decide what type of inhibitor is being used. Eo T I S V (g/I) (°C) (mmol/ml) (mmol/ml) (mmol/ml-min) 1.6 30 0.1 2.63 1.6 30 0.033 1.92 1.6 30 0.02 1.47 1.6 30 0.01 0.96 1.6 30 0.005 0.56 1.6 49.6 0.1 5.13 1.6 49.6 0.033 3.70 1.6 49.6 0.01 1.89 1.6 49.6 0.0067 1.43 1.6 49.6 0.005 1.11 0.92 30 0.1 1.64 0.92 30 0.02 0.90 0.92 30 0.01 0.58 0.92 30 0.6 0.1 1.33 0.92 30 0.6 0.033 0.80 0.92 30 0.6 0.02 0.57
- Why does a pure noncompetitive inhibitor not changethe observed KM?Consider the following experimental data from another experiment: [S] 1.5 2.00 2.50 5.00 10.00 V (No inhibitor) mmol ml¹ min¹ 0.167 0.204 0.232 0.313 0.385 V (inhibitor) mmol ml¹¹ min¹¹ 0.115 0.143 0.167 0.250 0.333 Calculate Km and V max and determine whether this inhibitor is competitive, non-competitive or uncompetitive.PTP1B Substrate kcat Km kcat/Km UM 10-7 x (s-1 M) DADEPYLIPQQG DADAPYLIPQQG DAAEPYLIPQQG AAAAPYLIPQQG 44.6 + 1.8 39.8 + 0.32 3.9 + 0.9 13.7 + 0.46 1.1 + 0.25 0.29 + 0.01 35.3 + 0.22 6.6 ± 0.22 0.53 + 0.02 34.7 + 0.25 52.7 ± 0.7 0.066 + 0.001 (d) (. ) The units for kcat/KM in the above are given according to standard scientific notation. On this basis what is the value of this kinetic parameter for the DADEPYLIPQQG substrate?
- What is DPI? What are the advantages of DPI over MDI? How drug is aerosolized from a DPI system? Please answer at your own words.PTP1B Substrate kcat Km. kcat/Km UM 10-7 x (s-1 M) DADEPYLIPQQG DADAPYLIPQQG DAAEP YLIPQQG AAAAPYLIPQQG 44.6 + 1.8 39.8 + 0.32 3.9 + 0.9 13.7 + 0.46 1.1 + 0.25 0.29 + 0.01 35.3 + 0.22 6.6 + 0.22 0.53 + 0.02 34.7 + 0.25 52.7 + 0.7 0.066 + 0.001 ) The units for kcat/KM in the above are given according to standard scientific notation. On this (d) ( basis what is the value of this kinetic parameter for the DADEPYLIPQQG substrate?A schematic representation of the enzyme IspD complexed to inhibitor 3, and a series of inhibitors 3-5 are shown below. Ala202 lle240 mwww NH NH Val263 ОН www HN N- lle177 HN 'N' CI 3 X = N 4 X = C-CN 5 X = C-COO IC50 274 µM IC50 140 nM IC50 35 nM NH2 HN Val266 N -N O-H---- N HN %3D Arg157 HN wwww lle265 Explain why structure 4 is a more potent inhibitor (lower IC50 value) than inhibitor 3 and why structure 5 is a much weaker inhibitor (higher IC50 value) than 3 and 4.
- 3. In this question you can do the graphs on your computer. A, Calculate the Vmax and Km in the presence and absence of the inhibitor. B. Determine what type of inhibitor has been added. C. Determine the rate in the absence of inhibitor when the substrate concentration is 100mM. [S]mM 2.3 3.2 4 8 10 1/[S] MM-1 0.44 0.31 0.25 0.125 0.1 SIN INHIBIDOR Vo μmol/sec 1/Vo sec/μmol 0.25 0.29 X 0.4 X 4 3.5 X 2.5 X CON INHIBIDOR Vo μmol/sec1/Vo sec/μmol X X 0.17 0.29 X X X 4 3.5 XExplain the relationship between the minimum inhibitory concentrationand the therapeutic index. What is optimum for each?Do not include zeros at the end of decimal numbers. The problems and drug orders are presented for practice only, and actual prescribed dosages factors. Order: Rocephin 500 mg IM Supply: Rocephin 1 gram Directions: Reconstitute with 2.1 mL of sterile water to yield 350 mg/mL Give: ML Moving to another question will save this response. Type here to search O E M 90 DELL