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- References V8 ish (U.S.) Q Search f4 Review A AB I Text Predictions: On View Help BIU Part 2: 15 Cell components seen Illumination (ii) Complete the table comparing the light and electron microscopes. The table should fit on a single A4 sheet. Specimen preparation Image formation Magnification Tell me what you want to do Resolution Editor Suggestions! Showing H O E C t Αν Α f6 (iii) Explain the difference between magnification and resolution. E✓ ✓ f7 ) Light Microscope f8 19 E Ev Co A Electron Microscope f10 (A. C.1.2) 0 f11 V 12 Comments ✓ Dictate + 10 Rain CC scrollWhy would the following medium not be considereda chemically defined medium: glucose, 5 grams (g);NH4Cl, 1 g; KH2PO4, 1 g; MgSO4, 0.3 g; yeast extract, 5 g;distilled water, 1 liter? What is aseptic technique andwhy is it necessary?A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…
- A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…A PhD student leaving for vacation has asked an undergraduate student to perform daily media changes forhis iPSCs while he is gone. The culture is happening in 12-well plates where a volume of 2 mL is optimal. OnSaturday, when changing the media, the undergraduate decides to add 4 ml of media to the dishes (insteadof 2 ml) because he wants to skip lab and watch the Super Bowl on Sunday. He decides to add twice thevolume of media (4 mL) to tide the cells over till Monday. However, when the graduate student returns onMonday, he finds that some of his cells have died. Your job is to determine whether the cells died due to alack of oxygen. For the calculations that follow, diffusion and reaction occurs only in one direction. Also,assume that reaction only occurs at the cell-media interface. Use Michaelis-Menten type kinetics for oxygenuptake. You may use the following information:Ps = 150 mmHg (ambient oxygen tension)K = 1.19 nmol / mL / mmHg (solubility of oxygen in medium)D = 2…Which of the following sample(s) is NOT expected to cause a SUBSTANTIAL decrease in absorbance over time when performing light scattering experiments with bacteria? [Choose the best possible answer] LOAD and Bacteria alone Lysozyme standards HEW and CARB1 Bacteria alone
- POTATO OSMOSIS (LABORATORY EXPERIMENTATION) MATERIALS: Small basin 2 medium size potatoes (fresh) Table salt Sugar Distilled water (Wilkins or H2Zero) PROCEDURE: A. POTATOES Cut the potatoes to half, making 4 pcs of potatoes Cook one of the half potatoes and set aside the 3 other potatoes Label the cooked potato as letter B. MAKING A CAVITY Make a cavity of each half potato Label the raw potato as A B and C In potato A place salt on the cavity In potato B place sugar in the cavity Leave the potato C empty. Place sugar on the cavity of potato D C. POTATO OSMOSIS - Place distilled water in the basin and arranged the potatoes inside the basin. Leave it for 1hr. Observe after an hour. Do not mind the color of the potato. QUESTIONS: 1. What is your observation from potato A to D? 2. What can you conclude by comparing potato A and potato B? 3. What can you conclude by comparing potato A and potato C?POTATO OSMOSIS (LABORATORY EXPERIMENTATION) MATERIALS: Small basin 2 medium size potatoes (fresh) Table salt Sugar Distilled water (Wilkins or H2Zero) PROCEDURE: A. POTATOES Cut the potatoes to half, making 4 pcs of potatoes Cook one of the half potatoes and set aside the 3 other potatoes Label the cooked potato as letter B. MAKING A CAVITY Make a cavity of each half potato Label the raw potato as A B and C In potato A place salt on the cavity In potato B place sugar in the cavity Leave the potato C empty. Place sugar on the cavity of potato D C. POTATO OSMOSIS - Place distilled water in the basin and arranged the potatoes inside the basin. Leave it for 1hr. Observe after an hour. Do not mind the color of the potato. QUESTIONS: 1. What can you conclude by comparing potato A and potato D? 2. The liquid in the cavity of some potatoes came from where? 3. What is your conclusion about the experiment?The figure above depicts an agar cube with a side length of 13\, \text{mm}13mm13, start text, m, m, end text. In an experiment, students submerged the cube in red dye for 121212 hours. The red dye permeated 1\, \text{mm}1mm1, start text, m, m, end text on each side, as indicated by the shading in the figure. Volume of a rectangular solid: V = lwhV=lwhV, equals, l, w, h Calculate the volume of the agar cube that remained unpenetrated by the red dye.
- 3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTESYou may want to use this resource for this problem. If you do, submit the output along with your solution.You have been given a confocal microscope equipped with the following lasers, excitation filters, andemission filters:Laser Emission filter355 nm 410-470 nm405 nm 470-500 nm488 nm 500-550 nm532 nm 570-610 nm561 nm 610-650 nm640 nm 660-700 nm808 nm 720-780 nmYour task is to design an experiment to visualize the following:1. Nuclei2. A fluorescent protein in the cytosol3. A cell membrane marker antibody conjugated with a fluorophore4. Actin filaments5. LysosomesYou may choose from the following fluorophores for each of the five channels:Nuclei Fluorescent protein Membrane marker Actin marker Lysosome trackerDAPI GFP FITC AF488 Phalloidin LysoTracker RedHoechst 33342 YFP WGA-TRITC AF568 Phalloidin LysoTracker DeepRedSYTO Deep Red RFP Cy7 AF594 Phalloidin LysoTracker Blue Part 3.1Choose appropriate fluorophores for each of the subcellular structures to be imaged, taking into…Which of the following samples are expected to cause a decrease in absorbance over time when performing light scattering experiments with bacteria? HEW, CARB 1 and Lysozyme standards Lysozyme standards and CARB 1 Lysozyme standards HEW, LOAD, and CARB1 You take 0.5 mL of Carb 1 and add it to 2.0 mL of buffer. You then add 7.5 mL Biuret to this sample. The total dilution of Carb 1 in the final sample is 9 20 10 0.05